Saltzman A G, Morse B, Whitman M M, Ivanshchenko Y, Jaye M, Felder S
Rhône-Poulenc Rorer Central Research, King of Prussia, PA 19406.
Biochem Biophys Res Commun. 1991 Dec 31;181(3):1469-78. doi: 10.1016/0006-291x(91)92105-s.
We report the cloning and the deduced amino acid sequence of cDNAs encoding both the human serotonin 5-HT2 and 5-HT1C receptors. The human 5-HT2 and 5-HT1C receptors shared 87% and 90% amino acid homology, respectively, with their rat counterparts. The most divergent regions of the 5-HT2 receptor between human and rat were the N-terminal extracellular domain (75% homology) and the C-terminal intracellular domain (67% homology between amino acids 426-474). The greatest variability between the human and rat 5-HT1C receptors were at the N-terminal extracellular domain (78% homology) and the third cytoplasmic loop (71% homology). The availability of the cloned human 5-HT2 and 5-HT1C receptors will help facilitate the further understanding of the molecular pharmacology and physiology of these receptors.
我们报告了编码人血清素5-HT2和5-HT1C受体的cDNA的克隆及推导的氨基酸序列。人5-HT2和5-HT1C受体与大鼠相应受体的氨基酸同源性分别为87%和90%。人与大鼠之间5-HT2受体差异最大的区域是N端细胞外结构域（同源性75%）和C端细胞内结构域（氨基酸426 - 474之间同源性67%）。人与大鼠5-HT1C受体之间差异最大的是N端细胞外结构域（同源性78%）和第三个细胞质环（同源性71%）。克隆的人5-HT2和5-HT1C受体的可得性将有助于进一步了解这些受体的分子药理学和生理学。
