Lai Zhibing, Faris Justin D, Weiland John J, Steffenson Brian J, Friesen Timothy L
Department of Plant Pathology, North Dakota State University, Fargo, ND 58105, USA.
Fungal Genet Biol. 2007 May;44(5):323-9. doi: 10.1016/j.fgb.2006.11.009. Epub 2007 Jan 16.
A Pyrenophora teres f. teres cross between isolates 0-1 and 15A was used to evaluate the genetics of avirulence associated with barley lines Canadian Lake Shore (CLS), Tifang, and Prato. 15A is avirulent on Tifang and CLS, but virulent on Prato. Conversely, 0-1 is avirulent on Prato, but virulent on Tifang and CLS. Avirulence:virulence on Tifang and CLS segregated 1:1, whereas avirulence:virulence on Prato segregated 3:1. An AFLP-based linkage map was constructed and used to identify a single locus derived from 15A (AvrHar) conferring avirulence to Tifang and CLS. Virulence on Prato was conferred by two epistatic genes (AvrPra1 and AvrPra2). AvrPra2 co-segregated with AvrHar, but the two genes from opposite parents conferred opposite reactions. This work provides the foundation for the isolation of these avirulence genes.
用大麦网斑病菌（Pyrenophora teres f. teres）的菌株0-1和15A之间的杂交来评估与大麦品种加拿大湖岸（CLS）、提方和普拉托相关的无毒力遗传学。15A对提方和CLS无毒力，但对普拉托有毒力。相反，0-1对普拉托无毒力，但对提方和CLS有毒力。在提方和CLS上，无毒力:毒力分离比为1:1，而在普拉托上，无毒力:毒力分离比为3:1。构建了基于扩增片段长度多态性（AFLP）的连锁图谱，并用于鉴定来自15A的一个单一位点（AvrHar），该位点赋予对提方和CLS的无毒力。对普拉托的毒力由两个上位性基因（AvrPra1和AvrPra2）赋予。AvrPra2与AvrHar共分离，但来自相反亲本的这两个基因赋予相反的反应。这项工作为分离这些无毒力基因奠定了基础。
