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[反相高效液相色谱法测定不同季节显齿蛇葡萄不同部位的蛇葡萄素]

[Determination of ampelopsin in the different parts of Ampelopsis grossedentata in different seasons by RP-HPLC].

作者信息

Wang Yan, Zhou Liling, Li Rui, Wang Ying

机构信息

Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405.

出版信息

Zhong Yao Cai. 2002 Jan;25(1):23-4.

PMID:12583237
Abstract

OBJECTIVE

To determine ampelopsin in the root, stem and leaf of Ampelopsis grossedentata in different seasons by RP-HPLC.

METHODS

The determination was carried out with ODS column (5 microns, 250 mm x 46 mm), using methanol-water-phosphoric acid(25:75:0.1) as the mobile phase at a flow rate of 1.0 ml/min and detected at the wavelength 289 nm.

RESULTS

Ampelopsin content was in the order of leaf > stem > root. Ampelopsin content in Ampelopsis grossedentata collected in April and May was the highest.

摘要

目的

采用反相高效液相色谱法（RP-HPLC）测定不同季节显齿蛇葡萄根、茎、叶中白藜芦醇的含量。

方法

采用ODS柱（5微米，250毫米×4.6毫米），以甲醇-水-磷酸（25:75:0.1）为流动相，流速为1.0毫升/分钟，检测波长为289纳米进行测定。

结果

白藜芦醇含量顺序为叶＞茎＞根。4月和5月采集的显齿蛇葡萄中白藜芦醇含量最高。

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