[反相高效液相色谱法测定不同季节显齿蛇葡萄不同部位的蛇葡萄素]
[Determination of ampelopsin in the different parts of Ampelopsis grossedentata in different seasons by RP-HPLC].
作者信息
Wang Yan, Zhou Liling, Li Rui, Wang Ying
机构信息
Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405.
出版信息
Zhong Yao Cai. 2002 Jan;25(1):23-4.
OBJECTIVE
To determine ampelopsin in the root, stem and leaf of Ampelopsis grossedentata in different seasons by RP-HPLC.
METHODS
The determination was carried out with ODS column (5 microns, 250 mm x 46 mm), using methanol-water-phosphoric acid(25:75:0.1) as the mobile phase at a flow rate of 1.0 ml/min and detected at the wavelength 289 nm.
RESULTS
Ampelopsin content was in the order of leaf > stem > root. Ampelopsin content in Ampelopsis grossedentata collected in April and May was the highest.
目的
采用反相高效液相色谱法（RP-HPLC）测定不同季节显齿蛇葡萄根、茎、叶中白藜芦醇的含量。
方法
采用ODS柱（5微米，250毫米×4.6毫米），以甲醇-水-磷酸（25:75:0.1）为流动相，流速为1.0毫升/分钟，检测波长为289纳米进行测定。
结果
白藜芦醇含量顺序为叶＞茎＞根。4月和5月采集的显齿蛇葡萄中白藜芦醇含量最高。
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