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通过逆转录聚合酶链反应和非荧光低密度微阵列对冠状病毒进行通用检测及在原型毒株水平上的鉴别。

Generic detection of coronaviruses and differentiation at the prototype strain level by reverse transcription-PCR and nonfluorescent low-density microarray.

作者信息

de Souza Luna Luciano Kleber, Heiser Volker, Regamey Nicolas, Panning Marcus, Drexler Jan Felix, Mulangu Sabue, Poon Leo, Baumgarte Sigrid, Haijema Bert Jan, Kaiser Laurent, Drosten Christian

机构信息

Clinical Virology Section, Bernhard Nocht Institute for Tropical Medicine, Bernhard Nocht Str. 74, 20359 Hamburg, Germany.

出版信息

J Clin Microbiol. 2007 Mar;45(3):1049-52. doi: 10.1128/JCM.02426-06. Epub 2007 Jan 17.

DOI:10.1128/JCM.02426-06
PMID:17229859
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1829107/
Abstract

A nonfluorescent low-cost, low-density oligonucleotide array was designed for detecting the whole coronavirus genus after reverse transcription (RT)-PCR. The limit of detection was 15.7 copies/reaction. The clinical detection limit in patients with severe acute respiratory syndrome was 100 copies/sample. In 39 children suffering from coronavirus 229E, NL63, OC43, or HKU1, the sensitivity was equal to that of individual real-time RT-PCRs.

摘要

设计了一种非荧光、低成本、低密度的寡核苷酸阵列，用于在逆转录（RT）-PCR后检测整个冠状病毒属。检测限为15.7拷贝/反应。严重急性呼吸综合征患者的临床检测限为100拷贝/样本。在39名感染冠状病毒229E、NL63、OC43或HKU1的儿童中，该阵列的灵敏度与单独的实时RT-PCR相同。

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