de Souza Luna Luciano Kleber, Heiser Volker, Regamey Nicolas, Panning Marcus, Drexler Jan Felix, Mulangu Sabue, Poon Leo, Baumgarte Sigrid, Haijema Bert Jan, Kaiser Laurent, Drosten Christian
Clinical Virology Section, Bernhard Nocht Institute for Tropical Medicine, Bernhard Nocht Str. 74, 20359 Hamburg, Germany.
J Clin Microbiol. 2007 Mar;45(3):1049-52. doi: 10.1128/JCM.02426-06. Epub 2007 Jan 17.
A nonfluorescent low-cost, low-density oligonucleotide array was designed for detecting the whole coronavirus genus after reverse transcription (RT)-PCR. The limit of detection was 15.7 copies/reaction. The clinical detection limit in patients with severe acute respiratory syndrome was 100 copies/sample. In 39 children suffering from coronavirus 229E, NL63, OC43, or HKU1, the sensitivity was equal to that of individual real-time RT-PCRs.
设计了一种非荧光、低成本、低密度的寡核苷酸阵列,用于在逆转录(RT)-PCR后检测整个冠状病毒属。检测限为15.7拷贝/反应。严重急性呼吸综合征患者的临床检测限为100拷贝/样本。在39名感染冠状病毒229E、NL63、OC43或HKU1的儿童中,该阵列的灵敏度与单独的实时RT-PCR相同。