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使用靶向23S rRNA的寡核苷酸探针通过菌落杂交鉴定乳酸球菌和肠球菌。

Identification of lactococci and enterococci by colony hybridization with 23S rRNA-targeted oligonucleotide probes.

作者信息

Betzl D, Ludwig W, Schleifer K H

机构信息

Department of Microbiology, Technical University of Munich, Federal Republic of Germany.

出版信息

Appl Environ Microbiol. 1990 Sep;56(9):2927-9. doi: 10.1128/aem.56.9.2927-2929.1990.

DOI:10.1128/aem.56.9.2927-2929.1990
PMID:2275539
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC184868/
Abstract

Specific sequences of 23S rRNA of Lactococcus lactis, Enterococcus faecalis, Enteroccus faecium, and Enterococcus malodoratus/Enterococcus avium were identified, and complementary oligonucleotide probes were synthesized. The specificity of the probes was evaluated by dot blot and colony hybridizations. The probes can be used for the specific detection and identification of colonies of the corresponding species in mixed cultures.

摘要

鉴定了乳酸乳球菌、粪肠球菌、屎肠球菌和恶臭肠球菌/鸟肠球菌23S rRNA的特定序列，并合成了互补寡核苷酸探针。通过斑点印迹和菌落杂交评估了探针的特异性。这些探针可用于在混合培养物中特异性检测和鉴定相应菌种的菌落。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cdc/184868/5a74a0b9bfe6/aem00090-0349-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cdc/184868/11dd1b562f77/aem00090-0348-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cdc/184868/99095404e918/aem00090-0349-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cdc/184868/5a74a0b9bfe6/aem00090-0349-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cdc/184868/11dd1b562f77/aem00090-0348-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cdc/184868/99095404e918/aem00090-0349-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cdc/184868/5a74a0b9bfe6/aem00090-0349-b.jpg

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