一种用于反馈抗性DAHP合酶的突变型ARO4基因，其导致酿酒酵母对邻氟-DL-苯丙氨酸产生抗性并过量生产β-苯乙醇。

A mutated ARO4 gene for feedback-resistant DAHP synthase which causes both o-fluoro-DL-phenylalanine resistance and beta-phenethyl-alcohol overproduction in Saccharomyces cerevisiae.

作者信息

Fukuda K, Watanabe M, Asano K, Ouchi K, Takasawa S

机构信息

Foods and Liquors Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Ibaraki, Japan.

出版信息

Curr Genet. 1991 Dec;20(6):453-6. doi: 10.1007/BF00334771.

DOI:10.1007/BF00334771

PMID:1723662

Abstract

o-Fluoro-DL-phenylalanine (OFP)-resistant mutants which overproduce beta-phenethyl-alcohol were isolated from a laboratory strain of Saccharomyces cerevisiae. Cells of one of the mutants accumulated tyrosine and phenylalanine 1.5-3 fold more than did wild-type cells. Its 3-deoxy-D-arabino-hepturosonate-7-phosphate (DAHP) synthase (EC 4.1.2.15), encoded by ARO4, was free from feedback inhibition by tyrosine. Genetic analysis revealed that the mutation was controlled by a single dominant gene, ARO4-OFP, encoding feedback-resistant DAHP synthase by tyrosine, and that this gene caused both the OFP resistance and beta-phenethyl-alcohol overproduction. This was supported by molecular genetic studies using cloned ARO4 both from the wild-type and its mutant strain.

摘要

从酿酒酵母的一个实验室菌株中分离出了对邻氟-DL-苯丙氨酸（OFP）具有抗性且过量产生β-苯乙醇的突变体。其中一个突变体的细胞积累的酪氨酸和苯丙氨酸比野生型细胞多1.5至3倍。由ARO4编码的其3-脱氧-D-阿拉伯庚酮糖酸-7-磷酸（DAHP）合酶（EC 4.1.2.15）不受酪氨酸的反馈抑制。遗传分析表明，该突变由单个显性基因ARO4-OFP控制，该基因编码对酪氨酸反馈抗性的DAHP合酶，并且该基因导致了对OFP的抗性和β-苯乙醇的过量产生。使用从野生型及其突变体菌株克隆的ARO4进行的分子遗传学研究支持了这一点。