Teshiba S, Furter R, Niederberger P, Braus G, Paravicini G, Hütter R
Mol Gen Genet. 1986 Nov;205(2):353-7. doi: 10.1007/BF00430450.
Regulation of the two isozymes of 3-deoxy-D-arabino-heptulosonate-7phosphate synthase (DAHP synthase; EC 4.1.2.15) encoded by the genes ARO3 and ARO4 of Saccharomyces cerevisiae was studied. Both genes were shown to respond equally well to the general control of amino acid biosynthesis. Strains with mutations in these two genes were obtained by selecting first for a single aro3 mutation and afterwards for a double aro3 aro4 mutation. Gene ARO3, coding for the phenylalanine-dependent isozyme of DAHP synthase was cloned on the 2 micron multicopy vector pJDB207 by complementation of mutation aro3-1 in yeast. The ARO3 gene, carried originally on a 9.6 kb BamHI fragment (plasmid pME541A), was subcloned on a 1.9 kb HindIII-XbaI fragment (plasmid pME543). A transcript of about 1.5 kb was shown to proceed from the HindIII towards the XbaI site. Expression from the 9.6 kb as well as from the 1.9 kb fragment was normal on a multicopy vector, since in both cases DAHP synthase levels of about 50-fold the wild-type level were observed.
对酿酒酵母基因ARO3和ARO4编码的3-脱氧-D-阿拉伯庚酮糖-7-磷酸合酶（DAHP合酶；EC 4.1.2.15）的两种同工酶的调控进行了研究。结果表明，这两个基因对氨基酸生物合成的一般调控反应同样良好。通过先选择单个aro3突变体，然后选择双突变体aro3 aro4，获得了这两个基因发生突变的菌株。编码依赖苯丙氨酸的DAHP合酶同工酶的基因ARO3，通过酵母中aro3-1突变的互补作用，克隆到2μm多拷贝载体pJDB207上。最初携带在9.6 kb BamHI片段（质粒pME541A）上的ARO3基因，亚克隆到1.9 kb HindIII-XbaI片段（质粒pME543）上。显示约1.5 kb的转录本从HindIII朝向XbaI位点进行。在多拷贝载体上，9.6 kb片段和1.9 kb片段的表达均正常，因为在这两种情况下，均观察到DAHP合酶水平约为野生型水平的50倍。
