ElSohly Laboratories, Inc., Oxford, Mississippi, United States of America.
National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, Mississippi, United States of America.
Planta Med. 2018 Mar;84(4):267-271. doi: 10.1055/s-0044-100798. Epub 2018 Jan 22.
A liquid chromatography-tandem mass spectrometry single-laboratory validation was performed for the detection and quantification of the 10 major cannabinoids of cannabis, namely, (-)--Δ-tetrahydrocannabinol, cannabidiol, cannabigerol, cannabichromene, tetrahydrocannabivarian, cannabinol, (-)--Δ-tetrahydrocannabinol, cannabidiolic acid, cannabigerolic acid, and Δ-tetrahydrocannabinolic acid-A, in the root extract of . Acetonitrile : methanol (80 : 20, v/v) was used for extraction; d-cannabidiol and d- tetrahydrocannabinol were used as the internal standards. All 10 cannabinoids showed a good regression relationship with > 0.99. The validated method is simple, sensitive, and reproducible and is therefore suitable for the detection and quantification of these cannabinoids in extracts of cannabis roots. To our knowledge, this is the first report for the quantification of cannabinoids in cannabis roots.
进行了液相色谱-串联质谱单实验室验证，用于检测和定量大麻的 10 种主要大麻素，即（-）-Δ-四氢大麻酚、大麻二酚、大麻萜酚、大麻色烯、四氢大麻酚、大麻醇、（-）-Δ-四氢大麻酚、大麻二酚酸、大麻萜酚酸和 Δ-四氢大麻酚酸-A，在 的根提取物中。乙腈：甲醇（80：20，v/v）用于提取；d-大麻二酚和 d-四氢大麻酚用作内标。所有 10 种大麻素均表现出良好的回归关系， > 0.99。验证后的方法简单、灵敏、重现性好，因此适合检测和定量大麻根提取物中的这些大麻素。据我们所知，这是大麻根中大麻素定量的首次报告。
