Petri G, Nyiredy S, Veszki P, Oroszlán P, Turiák G
SOTE Gyógynövény- és Drogismereti Intézet, Budapest.
Acta Pharm Hung. 1995 May;65(3):63-7.
A simple, isotherm packed column gas chromatographic method was developed for the quantitative determination of neutral cannabinoids using 4-androstene-3,17-dion as internal standard. In order to achieve the best resolution and to avoid the evaluation of the disturbing hydrocarbon peaks a method was developed using "tandem" column made of 3% OV-1 (90%) and 3% OV-17 (10%) stationary phases. The psychotropic cannabinoids delta 1-tetrahydrocannabinol (delta 1-THC) and delta 1(6)-tetrahydrocannabionol (delta 1(6)-THC), as well as, their main metabolites cannabidiol (CBD) and cannabichromene (CBC) were baseline separated except the cannabigerol (CBG) and cannabinol (CBN) pair, however for these compounds the separation was also satisfactory for the quantitative determinations. The Kováts retention indices were calculated for the most important cannabinoids and established the detection limits, respectively (20-50 ng range). The reproducibility was found excellent cv% = 1.06 for delta 1-THC and the analysis time was 55 minutes. The practical usefulness of the method was demonstrated by the comparative analyses on hashish- and fibre type hemps.
建立了一种简单的等温填充柱气相色谱法，以4-雄烯-3,17-二酮为内标定量测定中性大麻素。为了获得最佳分离度并避免对干扰性烃类峰进行评估，开发了一种使用由3% OV-1（90%）和3% OV-17（10%）固定相组成的“串联”柱的方法。精神活性大麻素δ1-四氢大麻酚（δ1-THC）和δ1(6)-四氢大麻酚（δ1(6)-THC），以及它们的主要代谢物大麻二酚（CBD）和大麻色烯（CBC），除了大麻萜酚（CBG）和大麻酚（CBN）这一对化合物外均实现了基线分离，不过对于这些化合物，其分离度对于定量测定来说也令人满意。计算了最重要的大麻素的科瓦茨保留指数，并分别确定了检测限（20 - 50 ng范围）。发现该方法的重现性极佳，δ1-THC的cv% = 1.06，分析时间为55分钟。通过对大麻和纤维型大麻的对比分析证明了该方法的实际实用性。
