应用脉冲场凝胶电泳技术分析及分离巨细胞病毒DNA
Analysis and isolation of cytomegalovirus DNA by field inversion gel electrophoresis.
作者信息
van den Berg F M, Jiwa M, Rook R, Geelen J L
机构信息
Department of Pathology, University of Amsterdam, The Netherlands.
出版信息
J Gen Virol. 1988 Mar;69 ( Pt 3):699-704. doi: 10.1099/0022-1317-69-3-699.
High molecular weight human cytomegalovirus (CMV) DNA was isolated from agarose-embedded infected human diploid cells by employing field inversion gel electrophoresis. A high yield of CMV DNA molecules was obtained within 1 week of infecting the cell culture. Labelling of the viral DNA with biotin by nick translation enabled the detection of CMV-infected cells in sections of paraffin-embedded human adrenal gland by in situ hybridization.
通过采用场反转凝胶电泳，从琼脂糖包埋的感染人二倍体细胞中分离出高分子量的人巨细胞病毒（CMV）DNA。在感染细胞培养物1周内获得了高产率的CMV DNA分子。通过缺口平移用生物素标记病毒DNA，能够通过原位杂交在石蜡包埋的人肾上腺切片中检测CMV感染的细胞。
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