Bhattarakosol P, Yoosook C, Matangkasombut P
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Microbiol Immunol. 1991;35(7):525-33. doi: 10.1111/j.1348-0421.1991.tb01583.x.
Intratypic variation among 94 isolates of herpes simplex virus type 2 (HSV-2) was investigated using 4 different monoclonal antibodies (MAbs). By neutralization test, these MAbs appeared to be directed to at least 2 distinct epitopes on the viral glycoprotein D (gD), i.e., 6G6.G9 and 6E8.F11 which did not require complement (C-MAb) and gD-105 and gD-110 whose neutralizing activities could be enhanced by complement (C+MAb). The C-MAb pairs each separately could detect significant intratypic variations among the isolates. Whether these variations also existed in the gD epitope(s) recognized by C+MAbs remains to be elucidated. The results suggested that intratypic variation occurred on at least one of the neutralizable (thus related to protective immunity) epitopes on gD of HSV-2.
利用4种不同的单克隆抗体（MAb）对94株2型单纯疱疹病毒（HSV-2）的型内变异进行了研究。通过中和试验，这些单克隆抗体似乎针对病毒糖蛋白D（gD）上至少2个不同的表位，即不需要补体的6G6.G9和6E8.F11（C-MAb）以及其中和活性可被补体增强的gD-105和gD-110（C+MAb）。每对C-MAb均可单独检测到分离株之间显著的型内变异。这些变异是否也存在于C+MAb识别的gD表位中仍有待阐明。结果表明，HSV-2的gD上至少一个可被中和的（因此与保护性免疫相关的）表位发生了型内变异。
