[CD90在甲状腺相关眼病眼眶成纤维细胞中的表达研究]

[The study of CD90 expressing in orbital fibroblasts of thyroid associated ophthalmopathy].

作者信息

Tang Li, Luo Qing-Li, Xia Qing-Jie, Chen Qing-Ying, Liu Chun-Ling, Tang Jian

机构信息

Department of Ophthalmology, West China Hospital, Sichuan University, Chengdu 610041, China.

出版信息

Sichuan Da Xue Xue Bao Yi Xue Ban. 2006 Nov;37(6):879-81.

PMID:17243294

Abstract

OBJECTIVE

To investigate whether there is heterogeneous antigen-specific CD90 expressing on the surface of cultured orbital fibroblasts, and comparatively to study the distribution and proportion of CD90 positive cells in thyroid associated ophthalmopathy (TAO) and normal orbital tissue.

METHODS

Human orbital fibroblasts were cultivated for normal control and TAO. Indirect immunofluorescence and flow cytometric analysis were applied to detect the levels of CD90 expression.

RESULTS

The CD90 antigen expressed in some of orbital fibroblasts sourced wherever from normal/TAO extraocular muscles or normal/TAO connective/adipose tissue, in which the CD90+ cell occupation was 82.95% +/- 6.49%, 80.83% +/- 7.14%, 64.55% +/- 4.45%, 59.20% +/- 1.19% respectively. There were more CD90+ fibroblasts in extraocular muscles than in connective/adipose tissue (P < 0.05), but no significant difference between normal control and TAO tissue (P > 0.05).

CONCLUSIONS

Orbital fibroblasts can be separated into CD90+ and CD90- subsets with respect to surface CD90 expression. The ratio of CD90+ and CD90- cells depends on their site and distribution in the orbit.

摘要

目的

研究培养的眼眶成纤维细胞表面是否存在异质性抗原特异性CD90表达，并比较研究甲状腺相关眼病（TAO）和正常眼眶组织中CD90阳性细胞的分布及比例。

方法

培养正常人及TAO患者的眼眶成纤维细胞作为正常对照和TAO对照。采用间接免疫荧光和流式细胞术分析检测CD90表达水平。

结果

来源于正常/TAO眼外肌或正常/TAO结缔组织/脂肪组织的部分眼眶成纤维细胞表达CD90抗原，其中CD90+细胞占有率分别为82.95%±6.49%、80.83%±7.14%、64.55%±4.45%、59.20%±1.19%。眼外肌中CD90+成纤维细胞多于结缔组织/脂肪组织（P<0.05），但正常对照组织与TAO组织之间无显著差异（P>0.05）。