Department of Ophthalmology, Chengdu Integrated TCM & Western Medicine Hospital/ Chengdu First People's Hospital, Chengdu, 610041, Sichuan Province, China.
Key Laboratory of Standardization of Chinese Medicine, Ministry of Education, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, Sichuan Province, China.
Exp Eye Res. 2020 Dec;201:108276. doi: 10.1016/j.exer.2020.108276. Epub 2020 Sep 28.
To observe the effect of Prunella vulgaris polysaccharides (PVP) on cultured orbit fibroblasts in vitro from patients with thyroid-associated ophthalmopathy (TAO). PVP at different concentrations were used to treat different groups of fibroblasts from TAO patients and normal persons. Dexamethasone (Dex) was used as a positive control drug, and interferon-γ (IFN-γ) was used as a positive stimulant. The effects of PVP on the proliferation of orbital fibroblasts, the secretion of hyaluronic acid (HA), the expression of intercellular adhesion molecule-1 (ICAM-1/CD54) and apoptosis in orbital fibroblasts were determined. The experimental results showed when the concentration of PVP was greater than 400 μg/mL, it could significantly inhibit the proliferation of orbital fibroblasts from patients with TAO (P < 0.05). However, no definite inhibitory effect was observed in the orbital fibroblasts from the normal people. Dex could significantly inhibit the proliferation of orbital fibroblasts from patients with TAO and the normal people (P < 0.05). In contrast, every concentration of IFN-γ could promote the orbital fibroblasts from patients with TAO and the normal people proliferation. No groups had statistically significant stimulatory effect on HA secretion by orbital fibroblasts from normal people (P > 0.05). But the Dex group, IFN-γ+PVP-1600 group and IFN-γ+Dex group could significantly inhibit the secretion of HA from orbital fibroblasts of TAO patients. And there were no groups had statistically significant stimulatory effect on the expression of ICAM-1/CD54 in orbital fibroblasts from TAO patients (P > 0.05). PVP and Dex at all concentrations could significantly promote orbital fibroblast co-cultured with IFN-γ apoptosis (P < 0.05). But without IFN-γ, PVP and Dex at all concentrations could only significantly promote orbital fibroblast from TAO patients apoptosis (P < 0.05). These results suggest that PVP exerts its therapeutic effect by inhibiting the proliferation of orbital fibroblasts and promoting the apoptosis of orbital fibroblasts in TAO patients. In addition, in this process, HA secretion is suppressed. But the participation of IFN-γ is required. This effect is similar to that of Dex. And in the MTT experiment, the efficacy of PVP showed selectivity for TAO patients. This is different from Dex. This may be a feature of PVP that deserves attention.
观察夏枯草多糖（PVP）对甲状腺相关眼病（TAO）患者体外培养的眼眶成纤维细胞的作用。用不同浓度的 PVP 处理 TAO 患者和正常人的不同眼眶成纤维细胞组。地塞米松（Dex）作为阳性对照药物，干扰素-γ（IFN-γ）作为阳性刺激物。测定 PVP 对眼眶成纤维细胞增殖、透明质酸（HA）分泌、细胞间黏附分子-1（ICAM-1/CD54）表达及眼眶成纤维细胞凋亡的影响。实验结果表明，当 PVP 浓度大于 400μg/ml 时，可明显抑制 TAO 患者眼眶成纤维细胞的增殖（P<0.05）。但正常人眼眶成纤维细胞未见明显抑制作用。Dex 可明显抑制 TAO 患者和正常人眼眶成纤维细胞的增殖（P<0.05）。相反，IFN-γ 的每个浓度均可促进 TAO 患者和正常人眼眶成纤维细胞的增殖。各组对正常人眼眶成纤维细胞 HA 分泌均无统计学显著刺激作用（P>0.05）。但 Dex 组、IFN-γ+PVP-1600 组和 IFN-γ+Dex 组可明显抑制 TAO 患者眼眶成纤维细胞 HA 的分泌。各组对 TAO 患者眼眶成纤维细胞 ICAM-1/CD54 表达均无统计学显著刺激作用（P>0.05）。PVP 和 Dex 各浓度均可明显促进与 IFN-γ 共培养的眼眶成纤维细胞凋亡（P<0.05）。但无 IFN-γ 时，PVP 和 Dex 各浓度仅可明显促进 TAO 患者眼眶成纤维细胞凋亡（P<0.05）。这些结果提示 PVP 通过抑制 TAO 患者眼眶成纤维细胞的增殖和促进其凋亡发挥治疗作用。此外，在此过程中抑制 HA 的分泌。但需要 IFN-γ 的参与。这种作用类似于 Dex。并且在 MTT 实验中，PVP 的疗效对 TAO 患者表现出选择性。这与 Dex 不同。这可能是 PVP 值得关注的一个特点。
