Cornish Peter V, Ha Taekjip
Department of Physics, University of Illinois, Urbana-Champaign, 1110 West Green Street, Urbana, Illinois 61801-3080, USA.
ACS Chem Biol. 2007 Jan 23;2(1):53-61. doi: 10.1021/cb600342a.
Single-molecule methods have revolutionized scientific research by rendering the investigation of once-inaccessible biological processes amenable to scientific inquiry. Several of the more established techniques will be emphasized in this Review, including single-molecule fluorescence microscopy, optical tweezers, and atomic force microscopy, which have been applied to many diverse biological processes. Serving as a taste of all the exciting research currently underway, recent examples will be discussed of translocation of RNA polymerase, myosin VI walking, protein folding, and enzyme activity. We will end by providing an assessment of what the future holds, including techniques that are currently in development.
单分子方法通过使对曾经难以触及的生物过程的研究变得适合科学探究，彻底改变了科学研究。本综述将重点介绍几种较为成熟的技术，包括单分子荧光显微镜、光镊和原子力显微镜，这些技术已被应用于许多不同的生物过程。作为对当前正在进行的所有令人兴奋的研究的一个介绍，将讨论RNA聚合酶易位、肌球蛋白VI行走、蛋白质折叠和酶活性的最新例子。我们将通过评估未来的发展方向来结束本文，包括目前正在开发的技术。
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