Cordova Juan Carlos, Das Dibyendu Kumar, Manning Harris W, Lang Matthew J
Department of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, TN, United States.
Department of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, TN, United States; Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN, United States.
Curr Opin Struct Biol. 2014 Oct;28:142-8. doi: 10.1016/j.sbi.2014.08.010. Epub 2014 Oct 17.
Single molecule force manipulation combined with fluorescence techniques offers much promise in revealing mechanistic details of biomolecular machinery. Here, we review force-fluorescence microscopy, which combines the best features of manipulation and detection techniques. Three of the mainstay manipulation methods (optical traps, magnetic traps and atomic force microscopy) are discussed with respect to milestones in combination developments, in addition to highlight recent contributions to the field. An overview of additional strategies is discussed, including fluorescence based force sensors for force measurement in vivo. Armed with recent exciting demonstrations of this technology, the field of combined single-molecule manipulation and single-molecule detection is poised to provide unprecedented views of molecular machinery.
单分子力操纵与荧光技术相结合，在揭示生物分子机器的机制细节方面具有很大的前景。在这里，我们回顾力荧光显微镜，它结合了操纵和检测技术的最佳特性。除了突出该领域的最新贡献外，还讨论了三种主要的操纵方法（光镊、磁镊和原子力显微镜）在联合发展中的里程碑。还讨论了其他策略的概述，包括用于体内力测量的基于荧光的力传感器。凭借该技术最近令人兴奋的演示，单分子操纵和单分子检测相结合的领域有望提供分子机器前所未有的视图。
