Department of Biology, University of Chicago, Chicago, Illinois 60637.
Genetics. 1983 Nov;105(3):559-79. doi: 10.1093/genetics/105.3.559.
Data are presented that associate three new markers with the uniparental linkage group in Chlamydomonas reinhardii. One of these, mutant 10-6C, is a genetic marker for the structural gene of the large subunit of ribulose bisphosphate carboxylase. These results provide the first direct link between the uniparental gene map and the physical map of chloroplast DNA. The other two markers, Dr2 (DCMU resistant) and 8-36C (deficient in photosystem II activity), map to a single locus. The data suggest that mixing in zygotic chloroplasts may not be complete so that input genomes do not have equal opportunities to recombine. The data are not compatible with simple linear or circular maps but can be explained on the basis of the known physical structure of chloroplast DNA.
有数据表明,在莱茵衣藻的单亲遗传群中存在三个新的标记物。其中之一,突变体 10-6C,是核酮糖二磷酸羧化酶大亚基结构基因的遗传标记。这些结果首次将单亲基因图谱与叶绿体 DNA 的物理图谱直接联系起来。另外两个标记物,Dr2(DCMU 抗性)和 8-36C(光合作用 II 活性缺陷),位于一个单一的基因座上。这些数据表明,合子叶绿体中的混合可能不完全,因此输入的基因组没有平等的机会进行重组。这些数据与简单的线性或圆形图谱不兼容,但可以基于已知的叶绿体 DNA 的物理结构来解释。