Department of Genetics, North Carolina State University, Raleigh, North Carolina 27650.
Genetics. 1979 Apr;91(4):723-42. doi: 10.1093/genetics/91.4.723.
A screen for allelic variants of the enzyme catalase indicated that the Cat(+) locus is essentially monomorphic in D. melanogaster. Segmental aneuploidy was used to screen the genome for a dosage-sensitive region for catalase activity. One region, 75D-78A on the polytene chromosome map of 3L, exhibited a hyperploid/euploid ratio of enzyme activity of 1.5. Further dissection localized the region to 75D-76A. We suggest that this region contains the structural locus for catalase in D. melanogaster.Simple methods have been developed using the specific inhibitor, 3-amino-1,2,4-triazole, for the direct analysis of rates of synthesis and degradation of the Cat(+) gene product. Based on kinetic studies of catalase synthesis in flies aneuploid and euploid for region 75D-76B, we suggest that these techniques can be readily applied to an examination of mutants that control the expression of the structural gene for catalase in Drosophila.
一种用于检测过氧化氢酶酶的等位基因变异的筛选方法表明，Cat(+) 基因座在黑腹果蝇中基本是单态的。片段性的非整倍体被用于筛选过氧化氢酶活性的剂量敏感区域。在多线染色体图谱上的 3L 上，有一个区域，75D-78A，表现出超倍体/二倍体酶活比为 1.5。进一步的细分将该区域定位在 75D-76A。我们认为这个区域包含了黑腹果蝇中过氧化氢酶的结构基因位点。已经开发出了使用特异性抑制剂 3-氨基-1,2,4-三唑的简单方法，用于直接分析 Cat(+) 基因产物的合成和降解率。基于对 75D-76B 区域的果蝇非整倍体和整倍体的过氧化氢酶合成的动力学研究，我们认为这些技术可以很容易地应用于检查控制果蝇过氧化氢酶结构基因表达的突变体。
