Nakamura T, Hashimoto N, Maeda Y, Ikeda T, Nakagawa H, Takagi K
Department of Orthopaedic Surgery, Kumamoto University, Medical School, Japan.
J Spinal Disord. 1990 Dec;3(4):288-92.
Lumbar yellow ligaments were obtained from 20 cases of lumbar spinal canal stenosis and 20 cases of degenerative olisthesis with slippage of more than 3 mm. The ligaments were stained with safranin-O, von Kossa, and immunohistochemical staining methods (S-100 protein). In the unstable group the safranin-O staining was more intense, microscopic ossification and chondroblasts were noted more frequently, and S-100 protein was more abundant. These findings show that instability of the lumbar spine accelerates degeneration and chondrometaplasia of the yellow ligament, which may lead to the enchondral ossification of the ligament.
从20例腰椎管狭窄症患者和20例退行性椎体滑脱超过3mm的患者中获取腰椎黄韧带。采用番红O染色、冯科萨染色及免疫组织化学染色法（S-100蛋白）对韧带进行染色。在不稳定组中，番红O染色更强烈，显微镜下观察到骨化和软骨母细胞更频繁，且S-100蛋白更丰富。这些结果表明，腰椎不稳会加速黄韧带的退变和软骨化生，这可能导致韧带的软骨内成骨。
