人脑脊液中内皮素-1和内皮素-3样免疫反应性

Endothelin-1- and endothelin-3-like immunoreactivity in human cerebrospinal fluid.

作者信息

Ando K, Hirata Y, Togashi K, Kawakami M, Marumo F

机构信息

Department of Medicine, Tokyo Medical and Dental University, Japan.

出版信息

J Cardiovasc Pharmacol. 1991;17 Suppl 7:S434-6. doi: 10.1097/00005344-199100177-00124.

DOI:10.1097/00005344-199100177-00124

PMID:1725403

Abstract

Using highly specific and sensitive radioimmunoassays (RIAs) for endothelin-1 (ET-1) and endothelin-3 (ET-3), we have determined the concentrations of ET-1- and ET-3-like immunoreactivities (LI) in human cerebrospinal fluid (CSF). The concentration of ET-3-LI (82.5 +/- 4.8 pg/ml) was about 1.5-fold greater than that of ET-1-LI (34.3 +/- 1.3 pg/ml). There was no significant correlation between ET-1-LI and ET-3-LI concentrations in human CSF (r = 0.44). Reverse-phase high-performance liquid chromatography (HPLC) revealed a single major component of ET-1-LI and ET-3-LI coeluting with authentic ET-1 and ET-3, respectively. It is suggested that ET-1 and ET-3 play a neuropeptide-like role in the central nervous system.

摘要

我们使用针对内皮素 -1（ET -1）和内皮素 -3（ET -3）的高特异性和高灵敏度放射免疫分析法（RIA），测定了人脑脊液（CSF）中ET -1和ET -3样免疫反应性（LI）的浓度。ET -3 -LI的浓度（82.5±4.8 pg/ml）约为ET -1 -LI浓度（34.3±1.3 pg/ml）的1.5倍。人CSF中ET -1 -LI和ET -3 -LI浓度之间无显著相关性（r = 0.44）。反相高效液相色谱法（HPLC）显示，ET -1 -LI和ET -3 -LI的单一主要成分分别与 authentic ET -1和ET -3共洗脱。提示ET -1和ET -3在中枢神经系统中发挥类似神经肽的作用。