Hirata Y, Matsunaga T, Ando K, Furukawa T, Tsukagoshi H, Marumo F
Department of Medicine, Tokyo Medical and Dental University, Japan.
Biochem Biophys Res Commun. 1990 Feb 14;166(3):1274-8. doi: 10.1016/0006-291x(90)91003-b.
By use of radioimmunoassay (RIA) specific for endothelin-1 (ET-1), we have studied whether ET-1-like immunoreactivity (LI) exists in human cerebrospinal fluid (CSF). Human CSF from patients with old cerebrovascular disease contained far greater amounts of ET-1-LI (28 +/- 1.3 pg/ml, n = 9) than normal human plasma. Reverse-phase HPLC coupled with ET-1 RIA revealed a single major ET-1-LI component in human CSF extract coeluting with standard ET-1. These data suggest ET-1 is a neuropeptide secreted from neural tissues in humans.
通过使用针对内皮素 -1（ET -1）的放射免疫分析法（RIA），我们研究了人脑脊液（CSF）中是否存在类似 ET -1 的免疫反应性（LI）。患有陈旧性脑血管疾病患者的人脑脊液中所含的 ET -1 -LI（28 ± 1.3 pg/ml，n = 9）比正常人血浆中的含量高得多。反相高效液相色谱法结合 ET -1 RIA 显示，人脑脊液提取物中与标准 ET -1 共洗脱的单一主要 ET -1 -LI 成分。这些数据表明 ET -1 是人类神经组织分泌的一种神经肽。
