Suzuki H, Sato S, Suzuki Y, Oka M, Tsuchiya T, Iino I, Yamanaka T, Ishihara N, Shimoda S
Department of Endocrinology, Dokkyo University School of Medicine, Tochigi, Japan.
Ann Med. 1990;22(4):233-6. doi: 10.3109/07853899009148932.
A radioimmunoassay method for endothelin was developed. Antisera raised against endothelin 1 showed significant crossreaction with endothelin 2 and 3 (45 and 13%, respectively). Considerable endothelin immunoreactivity was shown to be present in the cerebrospinal fluid of patients with a subarachnoid hemorrhage, ranging from 0.3 pmol/l cerebrospinal fluid to 4.5 pmol/l cerebrospinal fluid, though no endothelin immunoreactivity was observed in the cerebrospinal fluid of controls and patients with cerebral infarction, subdural haematoma or brain tumours. Endothelin immunoreactivity was also observed in two out of five cerebrospinal fluid samples from patients with cerebral bleeding. Reverse phase high performance liquid chromatography showed that the main immunoreactive component in cerebrospinal fluid appeared to elute at the same position. There was, however, an immunoreactive component which eluted at the same position as endothelin 3. These results may support the idea that endothelin immunoreactivity in the cerebrospinal fluid originate mainly from endothelial and neural tissues and that endothelin may contribute to the generation of the vasospasm often observed in subarachnoid hemorrhage, a conclusion based on the exceptionally high endothelin immunoreactivity in cerebrospinal fluid observed in patients with subarachnoid haemorrhage.
开发了一种内皮素的放射免疫测定方法。针对内皮素-1产生的抗血清与内皮素-2和-3显示出显著的交叉反应（分别为45%和13%）。蛛网膜下腔出血患者的脑脊液中显示出相当量的内皮素免疫反应性，范围从0.3 pmol/脑脊液到4.5 pmol/脑脊液，而在对照组以及脑梗死、硬膜下血肿或脑肿瘤患者的脑脊液中未观察到内皮素免疫反应性。在脑出血患者的五份脑脊液样本中有两份也观察到了内皮素免疫反应性。反相高效液相色谱显示脑脊液中的主要免疫反应成分似乎在相同位置洗脱。然而，有一个免疫反应成分在与内皮素-3相同的位置洗脱。这些结果可能支持以下观点：脑脊液中的内皮素免疫反应性主要源自内皮组织和神经组织，并且内皮素可能促成蛛网膜下腔出血中经常观察到的血管痉挛的发生，这一结论基于在蛛网膜下腔出血患者脑脊液中观察到的异常高的内皮素免疫反应性。
