Hemsén A, Lundberg J M
Department of Pharmacology, Karolinska Institutet, Stockholm, Sweden.
Regul Pept. 1991 Oct 1;36(1):71-83. doi: 10.1016/0167-0115(91)90196-n.
The distribution of endothelin (ET) peptides in the pig was studied in a variety of tissues using selective radioimmunoassays combined with reverse-phase high performance liquid chromatography (HPLC). The levels of ET-like immunoreactivity (LI) were overall relatively low. The highest levels of ET-LI were found in blood vessels, cerebral and coronary arteries containing 3190 +/- 910 and 1330 +/- 450 fmol/g, respectively. Veins generally contained higher levels of ET-LI per tissue weight than corresponding arteries. Peripheral sympathetic and sensory ganglia contained a higher concentration of ET-LI than the studied central nervous system (CNS) areas. In the CNS the highest concentration of ET-LI was found in a non-neuronal structure, the choroid plexus. The levels of ET-LI were also relatively high in the respiratory tract (100-400 fmol/g). In the heart, the endocardium contained the highest levels (190 +/- 44 fmol/g). In the kidney, the concentration of ET-LI was 3-fold higher in the medulla than in the cortex. In the gastrointestinal tract all levels were below 100 fmol/g, except for the colon which contained 120 +/- 50 fmol/g. The characterization of ET-LI in extracts of some of these tissues revealed that ET-1 dominated in the lung, spleen and hypothalamus while ET-3 and ET-1 were present in approximately equal amounts in renal medulla and thoracic spinal cord. The HPLC analysis provided no clear-cut evidence for significant presence of vasoactive intestinal contractor, ET-2 or big ET-1(1-39) in the lung, spleen, kidney, spinal cord or hypothalamus. It is concluded that mature ET-1 and ET-3 are the predominant ET peptides in peripheral tissues and CNS.
采用选择性放射免疫分析法结合反相高效液相色谱法（HPLC），研究了猪体内多种组织中内皮素（ET）肽的分布情况。ET样免疫反应性（LI）水平总体相对较低。ET-LI水平最高的是血管、脑动脉和冠状动脉，分别为3190±910和1330±450 fmol/g。通常，每单位组织重量的静脉中ET-LI水平高于相应动脉。外周交感神经节和感觉神经节中ET-LI的浓度高于所研究的中枢神经系统（CNS）区域。在CNS中，ET-LI浓度最高的是一种非神经元结构——脉络丛。呼吸道中的ET-LI水平也相对较高（100 - 400 fmol/g）。在心脏中，心内膜的ET-LI水平最高（190±44 fmol/g）。在肾脏中，髓质中ET-LI的浓度是皮质的3倍。在胃肠道中，除结肠含120±50 fmol/g外，其他部位的ET-LI水平均低于100 fmol/g。对其中一些组织提取物中ET-LI的特性分析表明，ET-1在肺、脾和下丘脑中占主导，而在肾髓质和胸段脊髓中ET-3和ET-1的含量大致相等。HPLC分析没有明确证据表明在肺、脾、肾、脊髓或下丘脑中存在大量血管活性肠收缩肽、ET-2或大ET-1(1 - 39)。结论是成熟的ET-1和ET-3是外周组织和CNS中主要的ET肽。
