Maxwell Lynn D, Williams Fionnuala, Gilmore Paula, Meenagh Ashley, Middleton Derek
Northern Ireland Regional Histocompatibility and Immunogenetics Laboratory, City Hospital, Belfast, Northern Ireland.
Hum Immunol. 2004 Jun;65(6):613-21. doi: 10.1016/j.humimm.2004.02.028.
A polymerase chain reaction sequence-specific oligonucleotide probe typing method identifying and distinguishing alleles of the KIR2DS4 gene has been established. The system is based on the specific amplification of a region of this gene, followed by hybridization with 11 sequence-specific oligonucleotide probes. The method has been applied to a healthy group of Northern Irish caucasian individuals, establishing frequencies of alleles of this locus within the local population. Furthermore, cell line DNA and families from the 13th International Histocompatibility Workshop, in addition to local families, have also been allele typed at the KIR2DS4 locus. Haplotype segregation, with respect to KIR2DS4 alleles, has been examined by using the local family data. Within all sample groups investigated, four KIR2DS4 alleles were identified, two of which are novel to this investigation.
已建立一种用于鉴定和区分KIR2DS4基因等位基因的聚合酶链反应序列特异性寡核苷酸探针分型方法。该系统基于对该基因一个区域的特异性扩增，随后与11种序列特异性寡核苷酸探针杂交。该方法已应用于一组健康的北爱尔兰白种人个体，确定了该基因座在当地人群中等位基因的频率。此外，第13届国际组织相容性研讨会的细胞系DNA和家族，以及当地家族，也已在KIR2DS4基因座进行了等位基因分型。利用当地家族数据研究了KIR2DS4等位基因的单倍型分离情况。在所有调查的样本组中，鉴定出四个KIR2DS4等位基因，其中两个是本研究中的新发现。
