在自然感染绵羊的肝脏和心脏中检测梅迪-维斯纳病毒。

Detection of maedi-visna virus in the liver and heart of naturally infected sheep.

作者信息

Brellou G D, Angelopoulou K, Poutahidis T, Vlemmas I

机构信息

Laboratory of Pathology, School of Veterinary Medicine, Aristotle University of Thessaloniki, Thessaloniki 54124, Greece.

出版信息

J Comp Pathol. 2007 Jan;136(1):27-35. doi: 10.1016/j.jcpa.2006.10.001. Epub 2007 Jan 25.

DOI:10.1016/j.jcpa.2006.10.001

PMID:17258227

Abstract

Maedi-visna virus (MVV) in sheep, which infects mainly cells of the monocyte/macrophage lineage, produces changes in the lung, mammary gland, brain and joints. In this study, however, the liver and heart of six naturally infected sheep were examined for the presence of the virus. MVV proviral DNA was demonstrated by polymerase chain reaction (PCR) analysis, and immunohistochemical examination revealed viral antigens in the cytoplasm of hepatocytes and cardiac myocytes. Although histopathological examination showed mild to moderate, chronic lymphocytic cholangiohepatitis and myocarditis and the presence of small lymphoid aggregates, the typical maedi lymphoproliferative lesions (lymphoid follicle-like structures of considerable size with germinal centres) were not seen in the liver and heart. These novel findings suggest that, although the macrophage is the main cell for productive viral replication, the liver and heart represent additional MVV targets.

摘要

绵羊梅迪-维斯纳病毒（MVV）主要感染单核细胞/巨噬细胞谱系的细胞，可导致肺部、乳腺、大脑和关节发生病变。然而，在本研究中，对6只自然感染绵羊的肝脏和心脏进行了病毒检测。通过聚合酶链反应（PCR）分析证实了MVV前病毒DNA的存在，免疫组织化学检查在肝细胞和心肌细胞的细胞质中发现了病毒抗原。尽管组织病理学检查显示存在轻度至中度的慢性淋巴细胞性胆管肝炎和心肌炎以及小淋巴样聚集物，但在肝脏和心脏中未发现典型的梅迪淋巴增生性病变（具有生发中心的相当大的淋巴滤泡样结构）。这些新发现表明，虽然巨噬细胞是病毒有效复制的主要细胞，但肝脏和心脏也是MVV的额外靶器官。