反义RNA介导的小酸溶性芽孢蛋白合成减少导致产气荚膜梭菌芽孢对湿热和紫外线辐射的抗性降低。
Antisense-RNA-mediated decreased synthesis of small, acid-soluble spore proteins leads to decreased resistance of clostridium perfringens spores to moist heat and UV radiation.
作者信息
Raju Deepa, Setlow Peter, Sarker Mahfuzur R
机构信息
Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, Oregon 97331, USA.
出版信息
Appl Environ Microbiol. 2007 Apr;73(7):2048-53. doi: 10.1128/AEM.02500-06. Epub 2007 Jan 26.
Abstract
Previous work has suggested that a group of alpha/beta-type small, acid-soluble spore proteins (SASP) is involved in the resistance of Clostridium perfringens spores to moist heat. However, this suggestion is based on the analysis of C. perfringens spores lacking only one of the three genes encoding alpha/beta-type SASP in this organism. We have now used antisense RNA to decrease levels of alpha/beta-type SASP in C. perfringens spores by approximately 90%. These spores had significantly reduced resistance to both moist heat and UV radiation but not to dry heat. These results clearly demonstrate the important role of alpha/beta-type SASP in the resistance of C. perfringens spores.
摘要
先前的研究表明，一组α/β型小的、酸溶性芽孢蛋白（SASP）参与了产气荚膜梭菌芽孢对湿热的抗性。然而，这一推测是基于对仅缺失该生物体中编码α/β型SASP的三个基因之一的产气荚膜梭菌芽孢的分析。我们现在利用反义RNA将产气荚膜梭菌芽孢中α/β型SASP的水平降低了约90%。这些芽孢对湿热和紫外线辐射的抗性显著降低，但对干热的抗性未降低。这些结果清楚地证明了α/β型SASP在产气荚膜梭菌芽孢抗性中的重要作用。
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