Schroeder Marc, Aichernig Nina, Guebitz Georg M, Kokol Vanja
Institute of Engineering Materials and Design, University of Maribor, Maribor, Slovenia.
Biotechnol J. 2007 Mar;2(3):334-41. doi: 10.1002/biot.200600209.
Upgrading of the surface characteristics could enhance the bulk properties of naturally abundant fiber-forming materials for better performance or create new value-added products. Laccase can induce cross-linkage and covalent coupling of low molecular weight compounds onto lignocellulosic surfaces. For this purpose the 38-kDa laccase from Trametes hirsuta was purified and characterized. The best conditions for laccase-induced coating of flax fibers were determined. This evaluation was based on the obtained coloration and color depth. A screening was carried out with different phenols for their potential as monomers for enzyme-catalyzed polymerization resulting in a coating with antibacterial performance. While all the methoxyphenols showed different coloration with weak fastness properties, bacterial growth of Bacillus subtilis and Staphylococcus aureus was reduced significantly using ferulic acid and hydroquinone. Using laccase-induced coupling and polymerization, multi-functionality of the lignocellulosic surface, such as coloration and antimicrobial performance, was achieved, which depended on the nature of the applied phenolic monomer.
提升材料表面特性能够增强天然丰富的成纤材料的整体性能，以实现更好的性能表现，或创造新的增值产品。漆酶可诱导低分子量化合物在木质纤维素表面发生交联和共价偶联。为此，对源自糙皮侧耳的38 kDa漆酶进行了纯化和表征。确定了漆酶诱导亚麻纤维涂层的最佳条件。该评估基于所获得的色泽和色深。对不同酚类作为酶催化聚合单体的潜力进行了筛选，从而得到具有抗菌性能的涂层。虽然所有甲氧基酚都呈现出不同的色泽，但色牢度较差，而使用阿魏酸和对苯二酚时，枯草芽孢杆菌和金黄色葡萄球菌的细菌生长显著减少。通过漆酶诱导的偶联和聚合反应，实现了木质纤维素表面的多功能性，如色泽和抗菌性能，这取决于所应用酚类单体的性质。
