用于生物用途的内毒素再纯化的简单方法。
Simple method for repurification of endotoxins for biological use.
作者信息
Tirsoaga Alina, Novikov Alexey, Adib-Conquy Minou, Werts Catherine, Fitting Catherine, Cavaillon Jean-Marc, Caroff Martine
机构信息
Equipe "Endotoxines," UMR 8619 du CNRS, IBBMC, Université de Paris-Sud, 91405 Orsay, France.
出版信息
Appl Environ Microbiol. 2007 Mar;73(6):1803-8. doi: 10.1128/AEM.02452-06. Epub 2007 Jan 19.
Abstract
A method for obtaining highly purified endotoxin (lipopolysaccharide [LPS]) in a few hours by repurification of commercial or laboratory preparations was devised. It avoids the use of phenol, which is not suitable for phenol-soluble lipopolysaccharides nor for some industrial purposes. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and matrix-assisted laser desorption ionization mass spectrometry analysis confirmed the integrity of the purified LPSs. The purified products did not activate Toll-like receptor 2 (TLR2), nuclear oligomerization domain 1 (NOD1), or NOD2 but did activate TLR4. Applied to different lipopolysaccharides, the method also improved their mass spectra, thus facilitating their structural analysis.
摘要
设计了一种通过对市售或实验室制备物进行再纯化在数小时内获得高度纯化内毒素（脂多糖 [LPS]）的方法。它避免使用苯酚，苯酚既不适用于酚溶性脂多糖，也不适用于某些工业用途。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和基质辅助激光解吸电离质谱分析证实了纯化的 LPS 的完整性。纯化产物不会激活 Toll 样受体 2（TLR2）、核寡聚化结构域 1（NOD1）或 NOD2，但会激活 TLR4。应用于不同的脂多糖时，该方法还改善了它们的质谱，从而便于其结构分析。
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本文引用的文献
1
TLR1- and TLR6-independent recognition of bacterial lipopeptides.
J Biol Chem. 2006 Apr 7;281(14):9049-57. doi: 10.1074/jbc.M512525200. Epub 2006 Feb 2.
2
Several uses for isobutyric Acid-ammonium hydroxide solvent in endotoxin analysis.
Appl Environ Microbiol. 1990 Jun;56(6):1957-9. doi: 10.1128/aem.56.6.1957-1959.1990.
3
Differential TLR recognition of leptospiral lipid A and lipopolysaccharide in murine and human cells.
J Immunol. 2005 Nov 1;175(9):6022-31. doi: 10.4049/jimmunol.175.9.6022.
4
Use of murine embryonic fibroblasts to define Toll-like receptor activation and specificity.
J Endotoxin Res. 2004;10(6):419-24. doi: 10.1179/096805104225006516.
5
Porphyromonas gingivalis lipopolysaccharide contains multiple lipid A species that functionally interact with both toll-like receptors 2 and 4.
Infect Immun. 2004 Sep;72(9):5041-51. doi: 10.1128/IAI.72.9.5041-5051.2004.
6
Lipid A components from Pseudomonas aeruginosa PAO1 (serotype O5) and mutant strains investigated by electrospray ionization ion-trap mass spectrometry.
J Mass Spectrom. 2004 May;39(5):505-13. doi: 10.1002/jms.611.
7
A methylated phosphate group and four amide-linked acyl chains in leptospira interrogans lipid A. The membrane anchor of an unusual lipopolysaccharide that activates TLR2.
J Biol Chem. 2004 Jun 11;279(24):25420-9. doi: 10.1074/jbc.M400598200. Epub 2004 Mar 24.
8
Stimulation of human Toll-like receptor (TLR) 2 and TLR6 with membrane lipoproteins of Mycoplasma fermentans induces apoptotic cell death after NF-kappa B activation.
Cell Microbiol. 2004 Feb;6(2):187-99. doi: 10.1046/j.1462-5822.2003.00356.x.
9
Structure of bacterial lipopolysaccharides.
Carbohydr Res. 2003 Nov 14;338(23):2431-47. doi: 10.1016/j.carres.2003.07.010.
10
Nod1 detects a unique muropeptide from gram-negative bacterial peptidoglycan.
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