Vasconcelos Laurylene César de Souza, Sampaio Fábio Correia, Sampaio Maria Carmélia Correia, Pereira Maria do Socorro Vieira, Higino Jane Sheila, Peixoto Maria Helena Pereira
Departament of Clinical and Social Dentistry, Federal University of Paraíba, João Pessoa, PB, Brazil.
Braz Dent J. 2006;17(3):223-7. doi: 10.1590/s0103-64402006000300009.
The purpose of this study was to investigate the antimicrobial effect of a Punica granatum Linn (pomegranate) phytotherapeutic gel and miconazole (Daktarin oral gel) against three standard streptococci strains (mutans ATCC 25175, sanguis ATCC 10577 and mitis ATCC 9811), S. mutans clinically isolated and Candida albicans either alone or in association. The effect of minimum inhibitory concentrations of the gels on the adherence of these microorganisms to glass was assessed in the presence of 5% sucrose, using increasing and doubled concentrations of the diluted solution of the gels ranging from 1:1 to 1:1024. The minimum inhibitory concentrations of adherence of Punica granatum L. gel against the test organisms were: 1:16 for S. mutans (ATCC), S. mutans (CI) and S. sanguis; 1:128 for S. mitis and 1:64 for C. albicans. The minimum inhibitory concentrations of adherence of miconazole against the same organisms were: 1:512, 1:64, 1:4, 1:128 and 1:16, respectively. In experiments with three and four associated microorganisms, the Punica granatum L. gel had greater efficiency in inhibiting microbial adherence than the miconazole. The results of this study suggest that this phytotherapeutic agent might be used in the control of adherence of different microorganisms in the oral cavity.
本研究的目的是调查石榴植物治疗凝胶和咪康唑（达克宁口腔凝胶）对三种标准链球菌菌株（变形链球菌ATCC 25175、血链球菌ATCC 10577和轻链球菌ATCC 9811）、临床分离的变形链球菌以及白色念珠菌单独或联合使用时的抗菌效果。在5%蔗糖存在的情况下，使用从1:1到1:1024的凝胶稀释溶液的递增和加倍浓度，评估凝胶的最低抑菌浓度对这些微生物粘附于玻璃的影响。石榴凝胶对受试微生物的最低抑菌粘附浓度分别为：对变形链球菌（ATCC）、变形链球菌（CI）和血链球菌为1:16；对轻链球菌为1:128，对白色念珠菌为1:64。咪康唑对相同微生物的最低抑菌粘附浓度分别为：1:512、1:64、1:4、1:128和1:16。在对三种和四种联合微生物进行的实验中，石榴凝胶在抑制微生物粘附方面比咪康唑具有更高的效率。本研究结果表明，这种植物治疗剂可能用于控制口腔中不同微生物的粘附。
