Dawson J, Sedgwick A D, Edwards J C, Lees P
Department of Pharmacology, Royal Veterinary College, Hatfield, Herts, England.
Int J Tissue React. 1991;13(4):171-85.
A murine 6-day air-pouch model of inflammation has been developed and used to compare the patterns of acute and chronic inflammatory response to three irritants, carrageenan, dextran and zymosan, each injected into the cavity of the pre-formed pouch. The inflammation was assessed by measurement of exudate volume and numbers of infiltrating leucocytes over a 30-day time course. Histological changes in the inflamed air-pouch lining tissue were also investigated. The inflammatory response to carrageenan was acute with moderate exudate formation and cell numbers. Dextran produced a mild inflammatory reaction with low cell infiltration into exudate. In contrast, the inflammatory response to zymosan was greater in terms of cell migration, but smaller in terms of exudate volume and occurred later in the time course. Histological changes in the inflamed air-pouch tissue were also markedly different in response to the three irritants. Carrageenan induced a rapid, mainly polymorphonuclear leucocyte (PMN) infiltrate into the tissue and deposition of fibrin on the luminal surface. The response to dextran was characterized by a rapid resolution of the inflammatory response, with fewer leucocytes present in the lining and no fibrin deposition. In contrast, zymosan caused a marked but slower leucocyte influx, with greater numbers of monocytes, and clearance of the zymosan particles from the air-pouch lining by macrophages. This study indicates that by using different irritants to produce inflammation, it may be possible to dissect the roles played by various cells and inflammatory mediators during acute and chronic inflammation.
已建立一种小鼠6天气囊炎症模型，并用于比较对三种刺激物（角叉菜胶、葡聚糖和酵母聚糖）的急性和慢性炎症反应模式，每种刺激物均注射到预先形成的气囊腔内。在30天的时间进程中，通过测量渗出液体积和浸润白细胞数量来评估炎症。还研究了发炎的气囊内衬组织的组织学变化。对角叉菜胶的炎症反应是急性的，伴有中等程度的渗出物形成和细胞数量增加。葡聚糖产生轻度炎症反应，渗出液中的细胞浸润较少。相比之下，对酵母聚糖的炎症反应在细胞迁移方面更大，但在渗出液体积方面较小，且发生在时间进程的后期。对三种刺激物的反应，发炎的气囊组织的组织学变化也明显不同。角叉菜胶诱导快速的、主要是多形核白细胞（PMN）浸润到组织中，并在腔表面沉积纤维蛋白。对葡聚糖的反应的特征是炎症反应迅速消退，内衬中白细胞较少，且无纤维蛋白沉积。相比之下，酵母聚糖引起明显但较慢的白细胞流入，单核细胞数量更多，巨噬细胞从气囊内衬清除酵母聚糖颗粒。这项研究表明，通过使用不同的刺激物来产生炎症，有可能剖析各种细胞和炎症介质在急性和慢性炎症过程中所起的作用。
