化学和物理组织固定方法的杀孢子活性。
Sporicidal activity of chemical and physical tissue fixation methods.
作者信息
Vardaxis N J, Hoogeveen M M, Boon M E, Hair C G
机构信息
Department of Medical Laboratory Science, Royal Melbourne Institute of Technology, Bundoora, Australia.
出版信息
J Clin Pathol. 1997 May;50(5):429-33. doi: 10.1136/jcp.50.5.429.
AIMS
The effects of alcohol based fixation and microwave stimulated alcohol fixation were investigated on spores of Bacillus stearothermophilus and Bacillus subtilis (var. niger).
METHODS
Spores were exposed to 10% formalin, or different concentrations of various alcohol containing fixatives (Kryofix/Spuitfix). Adequate controls were also set up in conjunction with the test solutions. The spores were immersed with and without adjunctive microwave stimulation in the various solutions tested. Possible surviving spores were recovered in revival broth and after incubation, and Gram staining viable counts were performed.
RESULTS
Alcohol based fixatives did not have a sporicidal effect on B stearothermophilus or B subtilis (var. niger) spores, and microwave stimulated alcohol fixation at 450 W and up to 75 degrees C did not have a sporicidal effect.
CONCLUSIONS
When alcohol based fixatives are used for fixation, precautions should be taken with the material thus treated, as it may contain viable spores or other pathogens, which are destroyed after 24 hours of formalin treatment. Of the physicochemical methods tested involving microwaving, none was successful in eliminating viable spores from the test material.
目的
研究酒精固定法和微波刺激酒精固定法对嗜热脂肪芽孢杆菌和枯草芽孢杆菌（黑色变种）孢子的影响。
方法
将孢子暴露于10%福尔马林或不同浓度的各种含酒精固定剂（Kryofix/Spuitfix）中。同时，结合测试溶液设置了适当的对照。在有和没有辅助微波刺激的情况下，将孢子浸入各种测试溶液中。在复苏肉汤中回收可能存活的孢子并孵育后，进行革兰氏染色活菌计数。
结果
酒精固定剂对嗜热脂肪芽孢杆菌或枯草芽孢杆菌（黑色变种）孢子没有杀菌作用，在450瓦及高达75摄氏度的微波刺激酒精固定也没有杀菌作用。
结论
当使用酒精固定剂进行固定时，应对如此处理的材料采取预防措施，因为它可能含有活孢子或其他病原体，经福尔马林处理24小时后会被消灭。在所测试的涉及微波处理的物理化学方法中，没有一种能成功从测试材料中消除活孢子。
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