Laboratory of Infectious Diseases, College of Veterinary Medicine, Jilin University, Changchun 130062, China; Key Laboratory of Zoonosis Research, Ministry of Education, Jilin University, Changchun 130062, China.
College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China.
Virus Res. 2017 Jun 15;238:1-7. doi: 10.1016/j.virusres.2017.05.011. Epub 2017 May 26.
Newcastle disease virus (NDV) can lead to a devastating disease to various avian species including pigeons. Genotype VI NDV is a major cause of Newcastle disease (ND) in Columbiformes (i.e. pigeons and doves). Here, we analyzed the genetic diversity of genotype VI based on the complete F gene sequences of 2 pigeon-origin isolates in northeastern China in 2015, along with 238 genotype VI strains available in GenBank. The phylogenetic tree and evolutionary distances revealed that the 2 new isolates were clustered into a new sub-genotype herein proposed as VIk. Although the 2 isolates contain the RQKRF cleavage site, a feature generally associated with virulent NDV strains, the values of ICPI and MDT showed lentogenic characteristics. The challenge experiment demonstrated that the isolate Pigeon/China/Jilin/NG05/2015 was pathogenic to pigeons, causing lesions in multiple tissues and organs. The emergence and spread of the sub-genotype VIk viruses illustrated that the genotype VI NDV was undergoing evolutionary changes. It is necessary to pay close attention and carry out epidemiological surveillance in pigeons.
新城疫病毒（NDV）可导致包括鸽子在内的各种禽类毁灭性疾病。基因型 VI NDV 是鸽形目鸟类（即鸽子和鸠鸽）中新城疫（ND）的主要病因。在这里，我们分析了 2015 年中国东北地区 2 株鸽源分离株基于完整 F 基因序列的基因型 VI 的遗传多样性，以及 GenBank 中 238 株基因型 VI 株。系统进化树和进化距离表明，这 2 个新分离株聚为一个新的亚基因型，我们将其命名为 VIk。尽管这 2 个分离株含有通常与强毒 NDV 株相关的 RQKRF 裂解位点，但 ICPI 和 MDT 值显示出低致病性特征。攻毒实验表明，分离株鸽/中国/吉林/NG05/2015 对鸽子具有致病性，可导致多种组织和器官出现病变。亚基因型 VIk 病毒的出现和传播表明基因型 VI NDV 正在发生进化变化。有必要密切关注并对鸽子进行流行病学监测。
