Molecular Bioscience-Parasitology, Institute of Agri-Food and Land Use, School of Biological Sciences, Queen's University Belfast, Belfast BT9 7BL, UK.
Trends Parasitol. 2011 Nov;27(11):505-13. doi: 10.1016/j.pt.2011.07.004. Epub 2011 Aug 30.
RNA interference (RNAi) has revolutionised approaches to gene function determination. From a parasitology perspective, gene function studies have the added dimension of providing validation data, increasingly deemed essential to the initial phases of drug target selection, pre-screen development. Notionally advantageous to those working on nematode parasites is the fact that Caenorhabditis elegans research spawned RNAi discovery and continues to seed our understanding of its fundamentals. Unfortunately, RNAi data for nematode parasites illustrate variable and inconsistent susceptibilities which undermine confidence and exploitation. Now well-ensconced in an era of nematode parasite genomics, we can begin to unscramble this variation.
RNA 干扰 (RNAi) 彻底改变了基因功能确定的方法。从寄生虫学的角度来看，基因功能研究增加了验证数据的维度，这对于药物靶点选择、预筛选开发的初始阶段越来越被认为是必不可少的。对研究线虫寄生虫的人来说，具有优势的是，秀丽隐杆线虫的研究催生了 RNAi 的发现，并继续为我们理解其基本原理提供依据。不幸的是，线虫寄生虫的 RNAi 数据显示出可变和不一致的易感性，这削弱了人们的信心和开发利用。现在线虫寄生虫基因组学已经深入人心，我们可以开始解开这种变化。
