Department of Public Health and Infectious Diseases, Sapienza University of Rome, 00185 Rome, Italy.
Genes (Basel). 2020 Jul 15;11(7):801. doi: 10.3390/genes11070801.
Advancements in technologies employed in high-throughput next-generation sequencing (NGS) methods are supporting the spread of studies that, combined with advances in computational biology and bioinformatics, have greatly accelerated discoveries within basic and biomedical research for many parasitic diseases. Here, we review the most updated "omic" studies performed on anisakid nematodes, a family of marine parasites that are causative agents of the fish-borne zoonosis known as anisakiasis or anisakidosis. Few deposited data on genomes are so far available, and this still hinders the deep and highly accurate characterization of biological aspects of interest, even as several transcriptomic and proteomic studies are becoming available. These have been aimed at discovering and characterizing molecules specific to peculiar developmental parasitic stages or tissues, as well as transcripts with pathogenic potential as toxins and allergens, with a broad relevance for a better understanding of host-pathogen relationships and for the development of reliable diagnostic tools.
高通量下一代测序（NGS）技术的进步支持了越来越多的研究的开展，这些研究结合计算生物学和生物信息学的进步，极大地加速了许多寄生虫病的基础和生物医学研究的发现。在这里，我们回顾了在海洋寄生虫旋毛虫科的最新“组学”研究，旋毛虫是一种海洋寄生虫，是一种被称为异尖线虫病或旋毛虫病的食源性人畜共患病的病原体。目前，只有少数基因组数据被存储，这仍然阻碍了对感兴趣的生物学方面的深入和高度精确的描述，尽管有几个转录组学和蛋白质组学研究正在进行。这些研究旨在发现和描述特定于特殊发育寄生虫阶段或组织的分子，以及具有作为毒素和过敏原的致病潜力的转录本，这对更好地理解宿主-病原体关系和开发可靠的诊断工具具有广泛的意义。
