Colin I, Berbinschi A, Denef J F, Ketelslegers J M
Unité de Diabétologie et Nutrition, Catholic University of Louvain, Medical School, Brussels, Belgium.
Endocrinology. 1992 Jan;130(1):544-6. doi: 10.1210/endo.130.1.1727722.
Endothelin-1 immunoreactivity (irET-1) was observed in rat and porcine thyroid glands. Using a radioimmunoassay for endothelin-1, the mean concentration in extracts of rat and porcine thyroid glands were 0.75 pg/mg +/- 0.03 (n = 4) and 1.5 pg/mg +/- 0.2 (n = 8) (mean +/- SE) respectively. Gel-filtration and reverse-phase HPLC showed that ir ET-1 eluted in a position identical to synthetic endothelin-1. In addition, immunohistochemical study showed that irET-1 is located within epithelial follicular cells. No immunostaining was seen in parafollicular C-cells nor in parathyroid.
在大鼠和猪的甲状腺中观察到内皮素-1免疫反应性（irET-1）。使用内皮素-1放射免疫分析法，大鼠和猪甲状腺提取物中的平均浓度分别为0.75 pg/mg ± 0.03（n = 4）和1.5 pg/mg ± 0.2（n = 8）（平均值 ± 标准误）。凝胶过滤和反相高效液相色谱显示，irET-1在与合成内皮素-1相同的位置洗脱。此外，免疫组织化学研究表明，irET-1位于上皮滤泡细胞内。在滤泡旁C细胞和甲状旁腺中未观察到免疫染色。
