Chakravarthy U, Douglas A J, Bailie J R, McKibben B, Archer D B
Department of Ophthalmology, Queen's University of Belfast, Ireland.
Invest Ophthalmol Vis Sci. 1994 Apr;35(5):2448-54.
To study the distribution of the endothelins in the eye using ocular tissues of human, rat, and porcine origin.
Extracts of ocular tissues were examined for immunoreactivity to endothelin 1 and 3 and pro-endothelin 1 using radioimmunoassay. Characterization of immunoreactivity was verified using high-performance liquid chromatography.
Immunoreactivity to endothelin 1 and endothelin 3 was found in all ocular tissues except the cornea, which contained no immunoreactivity to endothelin 3. Highest levels were found in the choroid, although species-related differences were present. Immunoreactivity to endothelin 3 was generally twofold to threefold higher than immunoreactivity to endothelin 1. The majority of endothelin-like-immunoreactivity in the retina was blood vessel-associated. High-pressure liquid chromatography (HPLC) confirmed that immunoreactive endothelin 1 and endothelin 3 in the tissue extracts eluted in identical positions to their respective standard synthetic peptides.
The endothelins are abundantly distributed in the eye. Endothelin 1 is present in its mature, 21 amino acid form, and only minimal amounts of the precursor pro-endothelin is found in ocular tissues. The wide distribution patterns point to complex roles for these peptides in blood vessel physiology and in such other functions as regulation of aqueous outflow, and in neurotransmission or modulation.
利用人、大鼠和猪源的眼组织研究内皮素在眼中的分布。
采用放射免疫分析法检测眼组织提取物对内皮素1、内皮素3和前内皮素1的免疫反应性。使用高效液相色谱法验证免疫反应性的特征。
除角膜对内皮素3无免疫反应性外，在所有眼组织中均发现了对内皮素1和内皮素3的免疫反应性。脉络膜中的含量最高，尽管存在种属差异。对内皮素3的免疫反应性通常比对内皮素1的免疫反应性高两到三倍。视网膜中大多数内皮素样免疫反应性与血管相关。高压液相色谱法（HPLC）证实，组织提取物中的免疫反应性内皮素1和内皮素3与各自的标准合成肽在相同位置洗脱。
内皮素在眼中广泛分布。内皮素1以其成熟的21个氨基酸形式存在，在眼组织中仅发现极少量的前体前内皮素。广泛的分布模式表明这些肽在血管生理学以及房水流出调节、神经传递或调节等其他功能中发挥着复杂的作用。
