Inagaki T, Kobayashi S, Ozeki N, Suzuki M, Fukuzawa Y, Shimizu K, Kato K, Kato K
First Department of Internal Medicine, Aichi Medical University, Japan.
Hepatology. 1992 Jan;15(1):46-53. doi: 10.1002/hep.1840150110.
Ultrastructural identification of light microscopic giant mitochondria was performed on the same specimens for light and electron microscopic observations. The liver tissue specimens were fixed in OsO4, embedded in epoxy resin, cut 4 microns thick and stained with polychrome. At the beginning of the study a light microscopic observation was made, and a microphotograph was taken. The identification of light microscopic giant mitochondria by conventional microscopy was identified by the occupation rate in liver cells, the negative findings of stainability and the morphological consistency (round, cigar-shaped and granular). The specimens were subsequently embedded again in epoxy resin and cut into ultrathin sections of 400 A. A transillumination electron microscope was used for the observation, and ultrastructural images of light microscopic giant mitochondria revealed that they were crystalloid bodies with a crystalline latticelike structure. The occupation rates within liver cells and the morphological shapes of the crystalloid bodies corresponded with those of light microscopic giant mitochondria. The light microscopic giant mitochondria obviously had different features from those of electron microscopic giant mitochondria and Mallory bodies (Yokoo's type II), although Mallory bodies showed the same staining properties as light microscopic giant mitochondria.
对用于光学显微镜和电子显微镜观察的相同标本进行了光学显微镜下巨大线粒体的超微结构鉴定。肝组织标本用四氧化锇固定，包埋在环氧树脂中，切成4微米厚，并用多色染色。在研究开始时进行了光学显微镜观察，并拍摄了显微照片。通过肝细胞中的占有率、染色性的阴性结果以及形态一致性（圆形、雪茄形和颗粒状），通过传统显微镜对光学显微镜下的巨大线粒体进行鉴定。随后将标本再次包埋在环氧树脂中，切成400埃的超薄切片。使用透射电子显微镜进行观察，光学显微镜下巨大线粒体的超微结构图像显示它们是具有晶体晶格状结构的晶体样体。肝细胞内的占有率和晶体样体的形态与光学显微镜下巨大线粒体的占有率和形态相对应。光学显微镜下的巨大线粒体明显具有与电子显微镜下的巨大线粒体和马洛里小体（横尾氏II型）不同的特征，尽管马洛里小体与光学显微镜下的巨大线粒体具有相同的染色特性。
