Sogo L F, Yaffe M P
Department of Biology, University of California, San Diego, La Jolla 92093-0347.
J Cell Biol. 1994 Sep;126(6):1361-73. doi: 10.1083/jcb.126.6.1361.
Yeast cells with the mdm10 mutation possess giant spherical mitochondria and are defective for mitochondrial inheritance. The giant mitochondria display classical features of mitochondrial ultrastructure, yet they appear incapable of movement or division. Genetic analysis indicated that the mutant phenotypes resulted from a single nuclear mutation, and the isolated MDM10 gene restored wild-type mitochondrial distribution and morphology when introduced into mutant cells. MDM10 encodes a protein of 56.2 kD located in the mitochondrial outer membrane. Depletion of Mdm10p from cells led to a condensation of normally extended, tubular mitochondria into giant spheres, and reexpression of the protein resulted in a rapid restoration of normal mitochondrial morphology. These results demonstrate that Mdm10p can control mitochondrial morphology, and that it plays a role in the inheritance of mitochondria.
具有mdm10突变的酵母细胞拥有巨大的球形线粒体，并且线粒体遗传存在缺陷。这些巨大的线粒体呈现出线粒体超微结构的经典特征，但它们似乎无法移动或分裂。遗传分析表明，突变表型是由单个核突变导致的，并且当将分离出的MDM10基因导入突变细胞时，可恢复野生型线粒体的分布和形态。MDM10编码一种位于线粒体外膜的56.2 kD蛋白质。从细胞中去除Mdm10p会导致正常延伸的管状线粒体凝聚成巨大的球体，而该蛋白质的重新表达会使线粒体形态迅速恢复正常。这些结果表明，Mdm10p可以控制线粒体形态，并且在线粒体遗传中发挥作用。
