Ma Ziyang, Gerton Jordan M, Wade Lawrence A, Quake Stephen R
Applied Physics and Physics, California Institute of Technology, Pasadena, California 91125, USA.
Phys Rev Lett. 2006 Dec 31;97(26):260801. doi: 10.1103/PhysRevLett.97.260801. Epub 2006 Dec 27.
We demonstrate apertureless near-field microscopy of single molecules at sub-10 nm resolution. With a novel phase filter, near-field images of single organic fluorophores were obtained with approximately sixfold improvement in the signal-to-noise ratio. The improvement allowed pairs of molecules separated by approximately 15 nm to be reliably and repeatedly resolved, thus demonstrating the first true Rayleigh resolution test for near-field images of single molecules. The potential of this technique for biological applications was demonstrated with an experiment that measured the helical rise of A-form DNA.
我们展示了分辨率低于10纳米的单分子无孔径近场显微镜技术。通过一种新型相位滤波器，获得了单个有机荧光团的近场图像，信噪比提高了约6倍。这种提高使得相隔约15纳米的分子对能够被可靠且重复地分辨出来，从而证明了对单分子近场图像的首次真正瑞利分辨率测试。通过测量A-DNA螺旋间距的实验，证明了该技术在生物应用方面的潜力。
