Chen Bin, Shi Jianjun, Zheng Junke, Chen Ying, Wang Kai, Yang Qingzhang, Chen Xuejin, Yang Zhuqing, Zhou Xiaofei, Zhu Youming, Chu Jianxin, Liu Ailian, Sheng Hui Z
Center for Developmental Biology, Xinhua Hospital, Shanghai Jiao Tong University, School of Medicine, 1665 Kong Jiang Road, Shanghai 200092, China.
Differentiation. 2007 Jun;75(5):350-9. doi: 10.1111/j.1432-0436.2006.00151.x. Epub 2007 Feb 5.
In previous studies, progenitor embryoid body-derived (EBD) cells have been derived from human embryonic germ cells. These cells express lineage markers of three primary germ layers, although their potential to produce true fetal cells of various types has yet to be tested. To this end, we have transplanted EBD cells into the fetal sheep liver. We show that these cells respond appropriately to environmental cues and give rise to hepatocytes and well-structured bile ducts. These results suggest that EBD cells are relatively uncommitted early progenitors capable of effective incorporation and differentiation in vivo. The ability to generate functional liver cells makes EBD cells potentially useful for cell therapy.
在先前的研究中，祖细胞来源的胚状体（EBD）细胞源自人类胚胎生殖细胞。这些细胞表达三个主要胚层的谱系标志物，尽管它们产生各种类型真正胎儿细胞的潜力尚未得到测试。为此，我们已将EBD细胞移植到胎羊肝脏中。我们发现这些细胞对环境信号作出了适当反应，并产生了肝细胞和结构良好的胆管。这些结果表明，EBD细胞是相对未定向的早期祖细胞，能够在体内有效整合和分化。生成功能性肝细胞的能力使EBD细胞在细胞治疗方面具有潜在用途。
