Teng Yincheng, Chen Bin, Tao Minfang
Department of Gynecology and Obstetrics, The 6th People's Hospital, School of Medicine, Shanghai Jiao Tong University, 600 Yishan Road, Shanghai 200233, People's Republic of China.
Biochem Biophys Res Commun. 2009 Dec 18;390(3):463-8. doi: 10.1016/j.bbrc.2009.09.094. Epub 2009 Sep 26.
We derived a cell population from cultured human primordial germ cells from early human embryos. The derivates, termed embryoid body-derived (EBD) cells, displayed an extensive capacity for proliferation and expressed a panel of markers in all three germ layers. Interestingly, EBD cells were also positive for markers of neural stem/progenitor cells, such as nestin and glial fibrillary acidic protein. When these cells were transplanted into the brain cavities of fetal sheep and postnatal NOD-SCID mice or nerve-degenerated tibialis anterior muscles, they readily gave rise to neurons or glial cells. To our knowledge, our data are the first to demonstrate that EBD cells can undergo further neurogenesis under suitable environments in vivo. Hence, with the abilities of extensive expansion, self-renewal, and differentiation, EBD cells may provide a useful donor source for neural stem/progenitor cells to be used in cell-replacement therapies for diseases of the nervous system.
我们从早期人类胚胎的培养人类原始生殖细胞中获得了一个细胞群体。这些衍生物被称为胚状体衍生（EBD）细胞，具有广泛的增殖能力，并在所有三个胚层中表达一组标志物。有趣的是，EBD细胞对于神经干细胞/祖细胞的标志物，如巢蛋白和胶质纤维酸性蛋白，也呈阳性。当将这些细胞移植到胎羊和出生后NOD-SCID小鼠的脑腔或神经退化的胫前肌中时，它们很容易分化为神经元或胶质细胞。据我们所知，我们的数据首次证明EBD细胞在体内合适的环境下可以进行进一步的神经发生。因此，凭借广泛扩增、自我更新和分化的能力，EBD细胞可能为用于神经系统疾病细胞替代疗法的神经干细胞/祖细胞提供有用的供体来源。
