Chang Ai-wu, Wu Yu-ming, Wu Yong-jun, Xu Dong
Center of Medical Experiment, Zhengzhou University, Zhengzhou 450052, China.
Wei Sheng Yan Jiu. 2006 Mar;35(2):159-61.
To develop the method of high performance liquid chromatography (HPLC) to determine the level of catecholamines (CAs) in 24h urinary, such as norepinephrine (NE), epinephrine (E), dopamine (DA), and to apply in the diagnosis of neuroblastoma (NB).
The determination was performed in a Zorbax SB-Aq-C18 (4.6 x 150 mm, 5 microm) column with a mobile phase of 0.02 mol/L KH2PO4 solution, and with fluorescence detector. In the condition, the levels of 24 h urinary NE, E, DA were detected by HPLC in healthy children, and in children with NB.
The recovery rates of NE, E and DA were 92.20%-94.38%, 94.60%-95.85% and 88.43%-91.68%, respectively, the corresponding with-day precisions (n=6) were 5.46%, 3.96% and 4.49%, respectively, and the between-day precisions (n=6) were 6.48%, 4.67% and 5.43%, respectively. The linear scopes of NE and E were 25-800 microg/L, respectively, the linear scope of DA was 50-1600 microg/L. The least detections were 2.5, 1.5, 0.5 microg/L, respectively.
This method is simple, accurate and high specificity, and can be used for the clinical diagnosis of NB.
建立高效液相色谱法（HPLC）测定24小时尿中去甲肾上腺素（NE）、肾上腺素（E）、多巴胺（DA）等儿茶酚胺（CAs）水平的方法，并应用于神经母细胞瘤（NB）的诊断。
采用Zorbax SB - Aq - C18（4.6×150 mm，5μm）色谱柱，以0.02 mol/L磷酸二氢钾溶液为流动相，荧光检测器检测。在此条件下，用HPLC检测健康儿童及NB患儿24小时尿中NE、E、DA水平。
NE、E、DA的回收率分别为92.20% - 94.38%、94.60% - 95.85%、88.43% - 91.68%，日内精密度（n = 6）分别为5.46%、3.96%、4.49%，日间精密度（n = 6）分别为6.48%、4.67%、5.43%。NE和E的线性范围分别为25 - 800μg/L，DA的线性范围为50 - 1600μg/L。最低检测限分别为2.5、1.5、0.5μg/L。
该方法简便、准确、特异性高，可用于NB的临床诊断。
