组蛋白在复制染色体上的沉积。
Deposition of histones onto replicating chromosomes.
作者信息
Jackson V, Granner D K, Chalkley R
出版信息
Proc Natl Acad Sci U S A. 1975 Nov;72(11):4440-4. doi: 10.1073/pnas.72.11.4440.
Abstract
The mode of distribution of newly synthesized and pre-existing histones has been studied during the process of chromosome replication. Newly synthesized histone was labeled with [3H]lysine and newly synthesized DNA was density labeled with iododeoxyuridine. The histone was covalently linked to DNA, and radiolabeled histone was analyzed on CsCl density gradients. We have defined conditions that do not give rise to histone randomization during isolation, and also developed a method of defining the distribution of histones in chromatin on a density gradient in the unavoidable presence of nonhistone protein. Three possible modes of distribution of histone onto the replicating chromosome can be conceived; we describe experiments designed to distinguish unequivocally among these possibilities and conclude that histones are deposited randomly onto the chromosome.
摘要
在染色体复制过程中，对新合成的组蛋白和已存在的组蛋白的分布模式进行了研究。用[³H]赖氨酸标记新合成的组蛋白，用碘脱氧尿苷对新合成的DNA进行密度标记。组蛋白与DNA共价连接，并在氯化铯密度梯度上分析放射性标记的组蛋白。我们已经确定了在分离过程中不会导致组蛋白随机化的条件，并且还开发了一种在不可避免地存在非组蛋白的情况下在密度梯度上确定染色质中组蛋白分布的方法。可以设想组蛋白在复制染色体上有三种可能的分布模式；我们描述了旨在明确区分这些可能性的实验，并得出结论：组蛋白随机沉积在染色体上。
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