组蛋白H4赖氨酸20位点的单甲基化促进染色质成熟。
Monomethylation of lysine 20 on histone H4 facilitates chromatin maturation.
作者信息
Scharf Annette N D, Meier Karin, Seitz Volker, Kremmer Elisabeth, Brehm Alexander, Imhof Axel
机构信息
Munich Centre of Integrated Protein Science and Adolf-Butenandt Institute, Ludwig Maximilians University of Munich, Schillerstr. 44, 80336 Munich, Germany.
出版信息
Mol Cell Biol. 2009 Jan;29(1):57-67. doi: 10.1128/MCB.00989-08. Epub 2008 Nov 10.
Abstract
Histone modifications play an important role in shaping chromatin structure. Here, we describe the use of an in vitro chromatin assembly system from Drosophila embryo extracts to investigate the dynamic changes of histone modifications subsequent to histone deposition. In accordance with what has been observed in vivo, we find a deacetylation of the initially diacetylated isoform of histone H4, which is dependent on chromatin assembly. Immediately after deposition of the histones onto DNA, H4 is monomethylated at K20, which is required for an efficient deacetylation of the H4 molecule. H4K20 methylation-dependent dl(3)MBT association with chromatin and the identification of a dl(3)MBT-dRPD3 complex suggest that a deacetylase is specifically recruited to the monomethylated substrate through interaction with dl(3)MBT. Our data demonstrate that histone modifications are added and removed during chromatin assembly in a highly regulated manner.
摘要
组蛋白修饰在塑造染色质结构中起着重要作用。在此，我们描述了利用果蝇胚胎提取物的体外染色质组装系统，来研究组蛋白沉积后组蛋白修饰的动态变化。与体内观察到的情况一致，我们发现最初二乙酰化的组蛋白H4亚型发生了去乙酰化，这依赖于染色质组装。组蛋白沉积到DNA上后，H4在K20位点立即发生单甲基化，这是H4分子高效去乙酰化所必需的。H4K20甲基化依赖的dl(3)MBT与染色质的结合以及dl(3)MBT-dRPD3复合物的鉴定表明，一种去乙酰化酶通过与dl(3)MBT相互作用而被特异性招募到单甲基化底物上。我们的数据表明，在染色质组装过程中，组蛋白修饰以高度调控的方式被添加和去除。
相似文献
1
Monomethylation of lysine 20 on histone H4 facilitates chromatin maturation.组蛋白H4赖氨酸20位点的单甲基化促进染色质成熟。
Mol Cell Biol. 2009 Jan;29(1):57-67. doi: 10.1128/MCB.00989-08. Epub 2008 Nov 10.
2
Purification and functional characterization of SET8, a nucleosomal histone H4-lysine 20-specific methyltransferase.核小体组蛋白H4赖氨酸20特异性甲基转移酶SET8的纯化及功能特性分析
Curr Biol. 2002 Jul 9;12(13):1086-99. doi: 10.1016/s0960-9822(02)00924-7.
3
The histone H4 lysine 20 monomethyl mark, set by PR-Set7 and stabilized by L(3)mbt, is necessary for proper interphase chromatin organization.组蛋白 H4 赖氨酸 20 位单甲基化标记由 PR-Set7 设置，并由 L(3)mbt 稳定，这对于适当的间期染色质组织是必要的。
PLoS One. 2012;7(9):e45321. doi: 10.1371/journal.pone.0045321. Epub 2012 Sep 14.
4
Set8 and L(3)mbt function in gene expression independently of histone H4 lysine 20 methylation.Set8 和 L(3)mbt 独立于组蛋白 H4 赖氨酸 20 甲基化发挥基因表达功能。
Genes Dev. 2024 Jun 25;38(9-10):455-472. doi: 10.1101/gad.351698.124.
5
PR-Set7 is a nucleosome-specific methyltransferase that modifies lysine 20 of histone H4 and is associated with silent chromatin.PR-Set7是一种核小体特异性甲基转移酶，可修饰组蛋白H4的赖氨酸20，并与沉默染色质相关。
Mol Cell. 2002 Jun;9(6):1201-13. doi: 10.1016/s1097-2765(02)00548-8.
6
Methylation of histone H4 lysine 20 by PR-Set7 ensures the integrity of late replicating sequence domains in Drosophila.PR-Set7介导的组蛋白H4赖氨酸20甲基化确保了果蝇中晚期复制序列结构域的完整性。
Nucleic Acids Res. 2016 Sep 6;44(15):7204-18. doi: 10.1093/nar/gkw333. Epub 2016 Apr 29.
7
Beyond histones: the elusive substrates of chromatin regulators.超越组蛋白：染色质调控因子的难以捉摸的底物。
Genes Dev. 2024 Jun 25;38(9-10):357-359. doi: 10.1101/gad.351969.124.
8
Free and chromatin-associated mono-, di-, and trimethylation of histone H4-lysine 20 during development and cell cycle progression.发育和细胞周期进程中组蛋白H4赖氨酸20的游离及与染色质相关的单甲基化、二甲基化和三甲基化。
Dev Biol. 2007 Apr 1;304(1):46-52. doi: 10.1016/j.ydbio.2006.12.019. Epub 2006 Dec 13.
9
Transition from a nucleosome-based to a protamine-based chromatin configuration during spermiogenesis in Drosophila.果蝇精子发生过程中从基于核小体的染色质构型向基于鱼精蛋白的染色质构型的转变。
J Cell Sci. 2007 May 1;120(Pt 9):1689-700. doi: 10.1242/jcs.004663.
10
Histone H4 lysine 20 mono-methylation directly facilitates chromatin openness and promotes transcription of housekeeping genes.组蛋白 H4 赖氨酸 20 单甲基化直接促进染色质开放性，并促进管家基因的转录。
Nat Commun. 2021 Aug 20;12(1):4800. doi: 10.1038/s41467-021-25051-2.
引用本文的文献
1
Acute multi-level response to defective chromatin assembly in S-phase.S期对有缺陷的染色质组装的急性多水平反应。
bioRxiv. 2024 Mar 27:2024.03.22.586291. doi: 10.1101/2024.03.22.586291.
2
Processivity and specificity of histone acetylation by the male-specific lethal complex.由雄性专一致死复合物介导的组蛋白乙酰化的连续性和特异性。
Nucleic Acids Res. 2024 May 22;52(9):4889-4905. doi: 10.1093/nar/gkae123.
3
Symmetric inheritance of parental histones contributes to safeguarding the fate of mouse embryonic stem cells during differentiation.亲本组蛋白的对称遗传有助于在分化过程中保护小鼠胚胎干细胞的命运。
Nat Genet. 2023 Sep;55(9):1555-1566. doi: 10.1038/s41588-023-01477-w. Epub 2023 Sep 4.
4
Role of Chromatin Replication in Transcriptional Plasticity, Cell Differentiation and Disease.染色质复制在转录可塑性、细胞分化和疾病中的作用。
Genes (Basel). 2022 Jun 2;13(6):1002. doi: 10.3390/genes13061002.
5
Chromatin and Nuclear Dynamics in the Maintenance of Replication Fork Integrity.染色质与核动力学在维持复制叉完整性中的作用
Front Genet. 2021 Dec 14;12:773426. doi: 10.3389/fgene.2021.773426. eCollection 2021.
6
Chromatin replication and epigenetic cell memory.染色质复制和表观遗传细胞记忆。
Nat Cell Biol. 2020 Apr;22(4):361-371. doi: 10.1038/s41556-020-0487-y. Epub 2020 Mar 30.
7
Distinct CoREST complexes act in a cell-type-specific manner.不同的 CoREST 复合物以细胞类型特异性的方式发挥作用。
Nucleic Acids Res. 2019 Dec 16;47(22):11649-11666. doi: 10.1093/nar/gkz1050.
8
Accurate Recycling of Parental Histones Reproduces the Histone Modification Landscape during DNA Replication.准确回收亲本组蛋白可在 DNA 复制过程中再现组蛋白修饰景观。
Mol Cell. 2018 Oct 18;72(2):239-249.e5. doi: 10.1016/j.molcel.2018.08.010. Epub 2018 Aug 23.
9
Cell-cycle regulation of non-enzymatic functions of the Drosophila methyltransferase PR-Set7.果蝇甲基转移酶 PR-Set7 的非酶功能的细胞周期调控。
Nucleic Acids Res. 2018 Apr 6;46(6):2834-2849. doi: 10.1093/nar/gky034.
10
Forks on the Run: Can the Stalling of DNA Replication Promote Epigenetic Changes?复制过程中的停滞：DNA复制的停滞会促进表观遗传变化吗？
Front Genet. 2017 Jun 22;8:86. doi: 10.3389/fgene.2017.00086. eCollection 2017.
本文引用的文献
1
Catalytic function of the PR-Set7 histone H4 lysine 20 monomethyltransferase is essential for mitotic entry and genomic stability.PR-Set7组蛋白H4赖氨酸20单甲基转移酶的催化功能对于有丝分裂进入和基因组稳定性至关重要。
J Biol Chem. 2008 Jul 11;283(28):19478-88. doi: 10.1074/jbc.M710579200. Epub 2008 May 14.
2
PR-Set7 establishes a repressive trans-tail histone code that regulates differentiation.PR-Set7建立了一种抑制性的跨尾组蛋白密码，该密码调节细胞分化。
Mol Cell Biol. 2008 Jul;28(14):4459-68. doi: 10.1128/MCB.00410-08. Epub 2008 May 12.
3
Histone H4 lysine 20 monomethylation promotes transcriptional repression by L3MBTL1.组蛋白H4赖氨酸20单甲基化通过L3MBTL1促进转录抑制。
Oncogene. 2008 Jul 17;27(31):4293-304. doi: 10.1038/onc.2008.67. Epub 2008 Apr 14.
4
Preferential dimethylation of histone H4 lysine 20 by Suv4-20.Suv4-20介导的组蛋白H4赖氨酸20优先二甲基化
J Biol Chem. 2008 May 2;283(18):12085-92. doi: 10.1074/jbc.M707974200. Epub 2008 Feb 21.
5
dCHD3, a novel ATP-dependent chromatin remodeler associated with sites of active transcription.dCHD3，一种与活跃转录位点相关的新型ATP依赖型染色质重塑因子。
Mol Cell Biol. 2008 Apr;28(8):2745-57. doi: 10.1128/MCB.01839-07. Epub 2008 Feb 4.
6
A class II histone deacetylase acts on newly synthesized histones in Tetrahymena.II类组蛋白去乙酰化酶作用于四膜虫中新合成的组蛋白。
Eukaryot Cell. 2008 Mar;7(3):471-82. doi: 10.1128/EC.00409-07. Epub 2008 Jan 4.
7
The histone methyltransferase SET8 is required for S-phase progression.组蛋白甲基转移酶SET8是S期进程所必需的。
J Cell Biol. 2007 Dec 31;179(7):1337-45. doi: 10.1083/jcb.200706150.
8
PR-Set7-dependent lysine methylation ensures genome replication and stability through S phase.PR-Set7 依赖性赖氨酸甲基化通过 S 期确保基因组复制和稳定性。
J Cell Biol. 2007 Dec 31;179(7):1413-26. doi: 10.1083/jcb.200706179. Epub 2007 Dec 24.
9
Structural basis for lower lysine methylation state-specific readout by MBT repeats of L3MBTL1 and an engineered PHD finger.L3MBTL1的MBT重复序列和工程化的PHD指蛋白对低赖氨酸甲基化状态特异性识别的结构基础
Mol Cell. 2007 Nov 30;28(4):677-91. doi: 10.1016/j.molcel.2007.10.023.
10
Certain and progressive methylation of histone H4 at lysine 20 during the cell cycle.细胞周期中组蛋白H4赖氨酸20位点发生的特定且渐进性的甲基化。
Mol Cell Biol. 2008 Jan;28(1):468-86. doi: 10.1128/MCB.01517-07. Epub 2007 Oct 29.
Zotero 插件