染色质蛋白质合成、DNA合成及脱氧核糖核蛋白组装的时间关系。
Temporal relationships of chromatin protein synthesis, DNA synthesis, and assembly of deoxyribonucleoprotein.
作者信息
Seale R L
出版信息
Proc Natl Acad Sci U S A. 1976 Jul;73(7):2270-4. doi: 10.1073/pnas.73.7.2270.
Abstract
Chromatin assembly has been investigated in terms of the sites on DNA where newly synthesized chromatin proteins associate. Chromatin from cells labeled with [14C]-BrdUrd and [3H]lysine was fixed with formaldehyde and resolved in CsCl gradients. By varying the spacing of the labeling intervals of the two isotopes so as to encompass all possible periods in S-phase, the association of labeled, newly synthesized proteins on newly synthesized (BrdUrd-substituted) or preexisting chromatin DNA was determined. In all experiments it was found that newly synthesized chromatin proteins predominantly associated with nonreplicating DNA. Possible mechanisms by which cells recycle preexisting chromatin proteins to restore the protein content of newly synthesized DNA are discussed.
摘要
染色质组装已根据新合成的染色质蛋白在DNA上的结合位点进行了研究。用[14C]-5-溴脱氧尿苷和[3H]赖氨酸标记的细胞中的染色质用甲醛固定，并在氯化铯梯度中分离。通过改变两种同位素标记间隔的间距，以涵盖S期的所有可能时期，确定了标记的新合成蛋白在新合成的（5-溴脱氧尿苷取代的）或预先存在的染色质DNA上的结合情况。在所有实验中都发现，新合成的染色质蛋白主要与非复制性DNA结合。文中讨论了细胞回收预先存在的染色质蛋白以恢复新合成DNA的蛋白质含量的可能机制。
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引用本文的文献
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