Hayashi Amanda Aparecida, de Medeiros Sérgio Raposo, Carvalho Marina Hojaij, Lanna Dante Pazzanese Duarte
Department of Animal Science, University of São Paulo - ESALQ, Piracicaba, SP - Brazil.
J Dairy Res. 2007 May;74(2):160-6. doi: 10.1017/S002202990600224X. Epub 2007 Feb 12.
Conjugated linoleic acid (CLA) has a range of biological properties, including effects on lipid metabolism, milk and body composition in animals. This study investigated the effects of dietary CLA on lactating rats and development of the suckling pups. Dams were fed either a control diet or the same diet supplemented with 25 g/kg of a fat supplement containing 540 g CLA/kg (final concentration of 13.5 g CLA/kg diet) from parturition to the 15th day post-partum. The CLA mixture used in this study contained the following isomers (per 100 g): cis-9, trans-11 (24 g); cis-10, trans-12 (35 g); cis-8, trans-10 (15 g); cis-11, trans-13 (17 g) and others (9 g). On d 15 post partum, CLA supplementation reduced milk fat content by 33% and pup growth by 21%. The milk fatty acid profile, with decreased content of short and medium chain acids, suggests CLA inhibition was more pronounced for de novo lipid synthesis. Consistent with these results, activities of fatty acid synthase, glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase were reduced by CLA treatment in the mammary gland and liver. In contrast, the activity of NADP-malate dehydrogenase was unchanged.
共轭亚油酸（CLA）具有一系列生物学特性，包括对动物脂质代谢、乳汁和身体组成的影响。本研究调查了日粮CLA对泌乳大鼠和哺乳幼崽发育的影响。从分娩到产后第15天，给母鼠喂食对照日粮或添加了25 g/kg脂肪补充剂（每千克含540 g CLA，日粮中CLA最终浓度为13.5 g/kg）的相同日粮。本研究中使用的CLA混合物含有以下异构体（每100 g）：顺式-9，反式-11（24 g）；顺式-10，反式-12（35 g）；顺式-8，反式-10（15 g）；顺式-11，反式-13（17 g）以及其他（9 g）。在产后第15天，补充CLA使乳脂含量降低了33%，幼崽生长降低了21%。乳脂肪酸谱中短链和中链酸含量降低，表明CLA对从头合成脂质的抑制作用更为明显。与这些结果一致，CLA处理降低了乳腺和肝脏中脂肪酸合酶、葡萄糖6-磷酸脱氢酶和6-磷酸葡萄糖酸脱氢酶的活性。相比之下，NADP-苹果酸脱氢酶的活性未发生变化。
