Echeverría María Gabriela, Díaz Silvina, Metz Germán Ernesto, Serena María Soledad, Panei Carlos Javier, Nosetto Edgardo
Virology, Faculty of Veterinary Sciences, National University of La Plata, 60 and 118, CC 296, 1900 La Plata, Argentina.
Virus Genes. 2007 Oct;35(2):313-20. doi: 10.1007/s11262-007-0081-4. Epub 2007 Feb 9.
We report the nucleotide sequence and genetic diversity of four Equine Arteritis Virus (EAV) ORF 5 and 6 from Argentina isolates, obtained from asymptomatic virus-shedding stallions. Nucleic acid recovered from the isolates were amplified by RT-PCR and sequenced. Nucleotide and deduced amino acid sequences from the Argentine isolates were compared with 17 sequences available from the GenBank. Phylogenetic analysis revealed that the Argentine isolates grouped together in a definite cluster near European strains. Despite the greater genetic variability among ORF 5 from different isolates and strains of EAV, phylogenetic trees based on ORF 5 and 6 are similar. Both trees showed that virus sequences from America and Europe segregate into distinct clades based on sequence analysis of either ORF 5 or 6. This study constitutes the first characterization of Argentine EAV isolates.
我们报告了从阿根廷无症状病毒脱落种马中获得的四株马动脉炎病毒（EAV）ORF 5和6的核苷酸序列及遗传多样性。从分离株中回收的核酸通过RT-PCR进行扩增并测序。将阿根廷分离株的核苷酸和推导的氨基酸序列与GenBank中可获得的17个序列进行比较。系统发育分析表明，阿根廷分离株在靠近欧洲毒株的一个明确簇中聚集在一起。尽管不同EAV分离株和毒株的ORF 5之间存在更大的遗传变异性，但基于ORF 5和6的系统发育树相似。两棵树均显示，基于ORF 5或6的序列分析，来自美洲和欧洲的病毒序列分为不同的进化枝。本研究构成了阿根廷EAV分离株的首次特征描述。
