Sá-Nunes Anderson, Medeiros Alexandra I, Sorgi Carlos A, Soares Edson G, Maffei Cláudia M L, Silva Célio L, Faccioli Lúcia H
Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Av. do Café s/no, Ribeirão Preto, São Paulo 14040-903, Brazil.
Microbes Infect. 2007 Oct;9(12-13):1393-401. doi: 10.1016/j.micinf.2006.10.007. Epub 2006 Dec 12.
Recent studies have shown the participation of Gr-1(+) cells in many types of infections; however, the role played by these cells in the immune response to fungal pathogens is controversial. In this study we determined whether Gr-1(+) cells are involved in the protective immune response in systemic Histoplasma capsulatum infection. Depletion of Gr-1(+) cells using the monoclonal antibody (MAb) RB6-8C5 increased histoplasmosis severity and inhibited the subsequent development of a protective immune response. In addition to the increased fungal burden in lungs and spleens, the Th1 response was found to be unbalanced in these mice and the suppression of the cellular immune response seemed to be associated with increased nitric oxide production. Taken together, these results indicate that Gr-1(+) cell depletion at the beginning of infection allows yeast multiplication and increases mice mortality. This study improves the understanding of the role of Gr-1(+) cells on the protective immunity in histoplasmosis.
近期研究表明，Gr-1(+)细胞参与了多种类型的感染；然而，这些细胞在针对真菌病原体的免疫反应中所起的作用存在争议。在本研究中，我们确定Gr-1(+)细胞是否参与系统性荚膜组织胞浆菌感染的保护性免疫反应。使用单克隆抗体（MAb）RB6-8C5清除Gr-1(+)细胞会加重组织胞浆菌病的严重程度，并抑制随后保护性免疫反应的发展。除了肺和脾中的真菌负荷增加外，还发现这些小鼠的Th1反应失衡，细胞免疫反应的抑制似乎与一氧化氮产生增加有关。综上所述，这些结果表明，感染初期Gr-1(+)细胞的清除会使酵母繁殖并增加小鼠死亡率。本研究增进了对Gr-1(+)细胞在组织胞浆菌病保护性免疫中作用的理解。
