NSCL的分子特征，NSCL是一个在发育中的神经系统中表达的编码螺旋-环-螺旋蛋白的基因。

Molecular characterization of NSCL, a gene encoding a helix-loop-helix protein expressed in the developing nervous system.

作者信息

Begley C G, Lipkowitz S, Göbel V, Mahon K A, Bertness V, Green A R, Gough N M, Kirsch I R

机构信息

Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia.

出版信息

Proc Natl Acad Sci U S A. 1992 Jan 1;89(1):38-42. doi: 10.1073/pnas.89.1.38.

We report here the molecular cloning and chromosomal localization of an additional member of the helix-loop-helix (HLH) family of transcription factors, NSCL. The NSCL gene was identified based on its hybridization to the previously described hemopoietic HLH gene, SCL. Murine NSCL cDNA clones were obtained from a day 11.5 mouse embryo cDNA library. The coding region is 399 base pairs and encodes a predicted protein of 14.8 kDa. The nucleotide sequence shows 71% identity and the amino acid sequence shows 61% identity to murine SCL in the HLH domain. The NSCL protein-coding region terminates six amino acids beyond the second amphipathic helix of the HLH domain. Expression of NSCL was detected in RNA from mouse embryos between 9.5 and 14.5 days postcoitus, with maximum levels of expression at 10.5-12 days. Examination of 12- and 13-day mouse embryos by in situ hybridization revealed expression of NSCL in the developing nervous system. The NSCL gene was mapped to murine chromosome 1. The very restricted pattern of NSCL expression suggests an important role for this HLH protein in neurological development.

我们在此报告转录因子螺旋-环-螺旋（HLH）家族另一个成员NSCL的分子克隆及染色体定位。NSCL基因是根据其与先前描述的造血HLH基因SCL的杂交情况而鉴定出来的。小鼠NSCL cDNA克隆是从11.5天的小鼠胚胎cDNA文库中获得的。编码区为399个碱基对，编码一个预测分子量为14.8 kDa的蛋白质。在HLH结构域中，核苷酸序列与小鼠SCL的一致性为71%，氨基酸序列的一致性为61%。NSCL蛋白编码区在HLH结构域第二个两亲性螺旋之后六个氨基酸处终止。在交配后9.5至14.5天的小鼠胚胎RNA中检测到NSCL的表达，在10.5至12天表达水平最高。通过原位杂交对12天和13天的小鼠胚胎进行检查，发现NSCL在发育中的神经系统中表达。NSCL基因被定位到小鼠1号染色体上。NSCL非常有限的表达模式表明该HLH蛋白在神经发育中起重要作用。