Wilusz J, Pettine S M, Shenk T
Princeton University, Department of Biology, NJ 08544.
Nucleic Acids Res. 1989 May 25;17(10):3899-908. doi: 10.1093/nar/17.10.3899.
We have constructed 14 independent point mutations in the conserved AAUAAA element of the SV40 late polyadenylation signal in order to study the recognition and function of alternative polyadenylation signals. A variant RNA containing an AUUAAA was polyadenylated at 20% the level of wild-type substrate RNA, while all other derivatives tested were not functional in vitro. The AUUAAA variant RNA formed specific complexes in native polyacrylamide gels and crosslinked to the AAUAAA-specific 64kd polypeptide, but at a lower efficiency than wild-type substrate RNA.