二氧化硅表面和玻璃微流控通道的“点击”修饰

"Click" modification of silica surfaces and glass microfluidic channels.

作者信息

Prakash Shaurya, Long Timothy M, Selby John C, Moore Jeffrey S, Shannon Mark A

机构信息

Department of Mechanical Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.

出版信息

Anal Chem. 2007 Feb 15;79(4):1661-7. doi: 10.1021/ac061824n.

DOI:10.1021/ac061824n

PMID:17297970

Abstract

This paper demonstrates a chemical surface modification method for covalent attachment of various polymers by using silane-based "click" chemistry on silica surfaces and within glass microchannels suitable for CE systems. Modified surfaces are characterized by contact angle measurements, X-ray photoelectron spectroscopy, and Fourier transform infrared-attenuated total reflection spectroscopy. Electroosmotic flow (EOF) measurements in modified and unmodified channels are provided. Spectroscopic and transport data show that various polymers can be covalently attached to glass surfaces with a measurable change in EOF.

摘要

本文展示了一种化学表面改性方法，该方法通过在适用于毛细管电泳（CE）系统的二氧化硅表面和玻璃微通道内使用基于硅烷的“点击”化学，实现各种聚合物的共价连接。通过接触角测量、X射线光电子能谱和傅里叶变换红外衰减全反射光谱对改性表面进行表征。提供了改性通道和未改性通道的电渗流（EOF）测量结果。光谱和传输数据表明，各种聚合物可以共价连接到玻璃表面，同时EOF会发生可测量的变化。